biostudies-literatureLin ZNatural Science Fund of Jiangsu ProvinceSocial development fund of Yangzhou municipalityNational Natural Science Foundation of ChinaCollaborative Fund of Yangzhou University and Yangzhou municipality1159-1173https://www.ebi.ac.uk/biostudies/studies/S-EPMC11028319biostudies-literatureUnknown67(7)Regulatory T cells play critical roles in self-tolerance and tumor evasion. CD4⁺NKG2D⁺ cells with regulatory activity are present in patients with NKG2DL⁺ tumors and juvenile systemic lupus erythematosus. We previously showed that TGF-β-producing CD4⁺NKG2D⁺ T cells are present in pCD86-Rae-1ε transgenic mice. Here, we performed both ex vivo and in vivo studies on pCD86-Rae-1ε transgenic mice and an MC38 tumor-bearing mouse model and show that NK1.1^-CD4⁺NKG2D⁺ T cells have regulatory activity in pCD86-Rae-1ε transgenic mice. Furthermore, this T-cell subset was induced in mice transplanted with NKG2DL⁺ tumor cells and produced TGF-β and FasL, and secreted low amounts of IFN-γ. This T-cell subseCancer immunology, immunotherapy : CIIRegulatory NK1.1^-CD4⁺NKG2D⁺ subset induced by NKG2DL⁺ cells promotes tumor evasion in mice.PMC1102831981273214BK2011449816715472012038-5BK20082158117278581471547YZ2014179Qian XHu CDing YZhang YXiao WJia XLin ZZhu GHan SQian LGong WfalseRegulatory NK1.1^-CD4⁺NKG2D⁺ subset induced by NKG2DL⁺ cells promotes tumor evasion in mice.Regulatory T cells play critical roles in self-tolerance and tumor evasion. CD4⁺NKG2D⁺ cells with regulatory activity are present in patients with NKG2DL⁺ tumors and juvenile systemic lupus erythematosus. We previously showed that TGF-β-producing CD4⁺NKG2D⁺ T cells are present in pCD86-Rae-1ε transgenic mice. Here, we performed both ex vivo and in vivo studies on pCD86-Rae-1ε transgenic mice and an MC38 tumor-bearing mouse model and show that NK1.1^-CD4⁺NKG2D⁺ T cells have regulatory activity in pCD86-Rae-1ε transgenic mice. Furthermore, this T-cell subset was induced in mice transplanted with NKG2DL⁺ tumor cells and produced TGF-β and FasL, and secreted low amounts of IFN-γ. This T-cell subse2018-01-01T00:00:00Z2018 Jul2025-04-22T20:40:31.833Z2025-04-06T03:17:03.529ZS-EPMC110283192980242610.1007/s00262-018-2172-6