biostudies-literatureForget MACanadian Institutes of Health Research1119-25https://www.ebi.ac.uk/biostudies/studies/S-EPMC11028683biostudies-literatureUnknown60(8)Polyfunctionality is the capacity of a T-cell to execute a variety of effector functions mainly mediated by production of cytokines, chemokines, and cytolytic enzymes. Studies in anti-viral immunity have acknowledged the importance of polyfunctionality in the clearance of infections and maintenance of protection. Although accepted in the field, this concept has not been as well characterized in cancer immunology. Here, we report the polyfunctionality profile analysis of a CD8(+) T-cell clone isolated from a lung cancer patient and directed against Dickkopf-1, a potentially new tumor-associated antigen (TAA). The clone showed Tc1/Th1 effector tendencies confirmed by secretion of cytokines such as IFN-γ, IP-10, MIP-1β, MIP-1α, IL-2, GM-CSF, and expression of cytolytic enzyme granzyme B. ThisCancer immunology, immunotherapy : CIIPolyfunctionality of a DKK1 self-antigen-specific CD8(+) T lymphocyte clone in lung cancer.PMC11028683MOP-62709Forget MAMartin JLapointe RTurcotte SReuben AfalsePolyfunctionality of a DKK1 self-antigen-specific CD8(+) T lymphocyte clone in lung cancer.Polyfunctionality is the capacity of a T-cell to execute a variety of effector functions mainly mediated by production of cytokines, chemokines, and cytolytic enzymes. Studies in anti-viral immunity have acknowledged the importance of polyfunctionality in the clearance of infections and maintenance of protection. Although accepted in the field, this concept has not been as well characterized in cancer immunology. Here, we report the polyfunctionality profile analysis of a CD8(+) T-cell clone isolated from a lung cancer patient and directed against Dickkopf-1, a potentially new tumor-associated antigen (TAA). The clone showed Tc1/Th1 effector tendencies confirmed by secretion of cytokines such as IFN-γ, IP-10, MIP-1β, MIP-1α, IL-2, GM-CSF, and expression of cytolytic enzyme granzyme B. This2011-01-01T00:00:00Z2011 Aug2025-04-22T20:48:19.332Z2025-04-06T03:16:30.399ZS-EPMC110286832168137410.1007/s00262-011-1055-x