biostudies-literatureUnknown61(10)Winkler CCytotoxic T lymphocytes (CTL) can kill Hodgkin's lymphoma (HL) cells, and CTL have been used for the treatment of Epstein-Barr virus (EBV)-positive HL. For patients with EBV-negative HL, this strategy cannot be employed and alternative target structures have to be defined. In order to establish a system for the stimulation of HL-reactive T cells, we used dendritic cells (DC) as antigen-presenting cells for autologous T cells and transfected these DC with RNA from established HL cell lines. After stimulation of peripheral blood mononuclear cells (PBMC) with RNA-transfected DC, we analyzed the reactivity of primed PBMC by interferon gamma enzyme-linked immunospot. Our results suggest the presence of antigens with expression in HL cell lines and recognition of these antigens in combination wiCancer immunology, immunotherapy : CII1769-79https://www.ebi.ac.uk/biostudies/studies/S-EPMC11029013biostudies-literatureHodgkin's lymphoma RNA-transfected dendritic cells induce cancer/testis antigen-specific immune responses.PMC11029013Schlaf GMax DAltermann WBanning-Eichenseer UKewitz SKornhuber MStaege MSSteingrube DSEmmer AWinkler CfalseHodgkin's lymphoma RNA-transfected dendritic cells induce cancer/testis antigen-specific immune responses.Cytotoxic T lymphocytes (CTL) can kill Hodgkin's lymphoma (HL) cells, and CTL have been used for the treatment of Epstein-Barr virus (EBV)-positive HL. For patients with EBV-negative HL, this strategy cannot be employed and alternative target structures have to be defined. In order to establish a system for the stimulation of HL-reactive T cells, we used dendritic cells (DC) as antigen-presenting cells for autologous T cells and transfected these DC with RNA from established HL cell lines. After stimulation of peripheral blood mononuclear cells (PBMC) with RNA-transfected DC, we analyzed the reactivity of primed PBMC by interferon gamma enzyme-linked immunospot. Our results suggest the presence of antigens with expression in HL cell lines and recognition of these antigens in combination wi2012-01-01T00:00:00Z2012 Oct2026-06-01T23:56:27.046Z2026-05-24T03:07:36.012ZS-EPMC110290132241937110.1007/s00262-012-1239-z