{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Porat-Dahlerbruch G"],"funding":["NIAID NIH HHS","National Institutes of Health","NIAID"],"pagination":["107649"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11031345"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["361"],"pubmed_abstract":["Biomolecular solid-state magic angle spinning (MAS) NMR spectroscopy frequently relies on selective <sup>13</sup>C-<sup>15</sup>N magnetization transfers, for various kinds of correlation experiments. Introduced in 1998, spectrally induced filtering in combination with cross polarization (SPECIFIC-CP) is a selective heteronuclear magnetization transfer experiment widely used for biological applications. At MAS frequencies below 20 kHz, commonly used for <sup>13</sup>C-detected MAS NMR experiments, SPECIFIC-CP transfer between amide <sup>15</sup>N and <sup>13</sup>C<sup>α</sup> atoms (NCA) is typically performed with radiofrequency (rf) fields set higher than the MAS frequency for both <sup>13</sup>C and <sup>15</sup>N channels, and high-power <sup>1</sup>H decoupling rf field is simultaneously applied. Here, we experimentally explore a broad range of NCA zero-quantum (ZQ) SPECIFIC-CP matching conditions at the MAS frequency of 14 kHz and compare the best high- and low-power matching conditions with respect to selectivity, robustness, and sensitivity at lower <sup>1</sup>H decoupling rf fields. We show that low-power NCA SPECIFIC-CP matching condition gives rise to 20% sensitivity enhancement compared to high-power conditions, in 2D NCA spectra of microcrystalline assemblies of HIV-1 CA<sub>CTD</sub>-SP1 protein with inositol hexakis-phosphate (IP6)."],"journal":["Journal of magnetic resonance (San Diego, Calif. : 1997)"],"pubmed_title":["High-efficiency low-power &lt;sup&gt;13&lt;/sup&gt;C-&lt;sup&gt;15&lt;/sup&gt;N cross polarization in MAS NMR."],"pmcid":["PMC11031345"],"funding_grant_id":["U54 AI170791","U54AI170791"],"pubmed_authors":["Polenova T","Porat-Dahlerbruch G","Struppe J"],"additional_accession":[]},"is_claimable":false,"name":"High-efficiency low-power &lt;sup&gt;13&lt;/sup&gt;C-&lt;sup&gt;15&lt;/sup&gt;N cross polarization in MAS NMR.","description":"Biomolecular solid-state magic angle spinning (MAS) NMR spectroscopy frequently relies on selective <sup>13</sup>C-<sup>15</sup>N magnetization transfers, for various kinds of correlation experiments. Introduced in 1998, spectrally induced filtering in combination with cross polarization (SPECIFIC-CP) is a selective heteronuclear magnetization transfer experiment widely used for biological applications. At MAS frequencies below 20 kHz, commonly used for <sup>13</sup>C-detected MAS NMR experiments, SPECIFIC-CP transfer between amide <sup>15</sup>N and <sup>13</sup>C<sup>α</sup> atoms (NCA) is typically performed with radiofrequency (rf) fields set higher than the MAS frequency for both <sup>13</sup>C and <sup>15</sup>N channels, and high-power <sup>1</sup>H decoupling rf field is simultaneously applied. Here, we experimentally explore a broad range of NCA zero-quantum (ZQ) SPECIFIC-CP matching conditions at the MAS frequency of 14 kHz and compare the best high- and low-power matching conditions with respect to selectivity, robustness, and sensitivity at lower <sup>1</sup>H decoupling rf fields. We show that low-power NCA SPECIFIC-CP matching condition gives rise to 20% sensitivity enhancement compared to high-power conditions, in 2D NCA spectra of microcrystalline assemblies of HIV-1 CA<sub>CTD</sub>-SP1 protein with inositol hexakis-phosphate (IP6).","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2025-07-02T03:04:47.736Z","creation":"2025-07-02T03:04:47.736Z"},"accession":"S-EPMC11031345","cross_references":{"pubmed":["38452523"],"doi":["10.1016/j.jmr.2024.107649"]}}