biostudies-literatureXu YNational Natural Science Foundation of China302https://www.ebi.ac.uk/biostudies/studies/S-EPMC11031446biostudies-literatureUnknown108(1)Alternative splicing (AS) greatly expands the protein diversity in eukaryotes. Although AS variants have been frequently reported existing in filamentous fungi, it remains unclear whether lignocellulose-degrading enzyme genes in industrially important fungi undergo AS events. In this work, AS events of lignocellulose-degrading enzymes genes in Aspergillus niger under two carbon sources (glucose and wheat straw) were investigated by RNA-Seq. The results showed that a total of 23 out of the 56 lignocellulose-degrading enzyme genes had AS events and intron retention was the main type of these AS events. The AS variant enzymes from the annotated endo-β-1,4-xylanase F1 gene (xynF1) and the endo-β-1,4-glucanase D gene (eglD), noted as XYNF1-AS and EGLD-AS, were characterized compared to their noApplied microbiology and biotechnologyAlternative splicing analysis of lignocellulose-degrading enzyme genes and enzyme variants in Aspergillus niger.PMC1103144632171476Liu XDong FWang RAjmal MLin HChen HXu YfalseAlternative splicing analysis of lignocellulose-degrading enzyme genes and enzyme variants in Aspergillus niger.Alternative splicing (AS) greatly expands the protein diversity in eukaryotes. Although AS variants have been frequently reported existing in filamentous fungi, it remains unclear whether lignocellulose-degrading enzyme genes in industrially important fungi undergo AS events. In this work, AS events of lignocellulose-degrading enzymes genes in Aspergillus niger under two carbon sources (glucose and wheat straw) were investigated by RNA-Seq. The results showed that a total of 23 out of the 56 lignocellulose-degrading enzyme genes had AS events and intron retention was the main type of these AS events. The AS variant enzymes from the annotated endo-β-1,4-xylanase F1 gene (xynF1) and the endo-β-1,4-glucanase D gene (eglD), noted as XYNF1-AS and EGLD-AS, were characterized compared to their no2024-01-01T00:00:00Z2024 Apr2026-06-01T21:19:51.953Z2026-05-21T03:08:11.689ZS-EPMC110314463863979610.1007/s00253-024-13137-y