{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Rudokas V"],"pubmed_abstract":["<h4>Background</h4>Allergen extracts and recombinant allergens are used in allergy diagnostics and immunotherapy. Since allergen extracts from different manufacturers lack proper standardization regarding their composition, monoclonal antibodies (MAbs) against specific allergen components can be used for their identification and quantification in allergen extracts. This study aimed to generate MAbs against allergen Der p 21 of <i>Dermatophagoides pteronyssinus</i> for the analysis of allergen extracts.<h4>Methods</h4>Recombinant Der p 21 was expressed in <i>E. coli</i> and purified using affinity chromatography. MAbs against Der p 21 were generated using hybridoma technology. House dust mite (HDM) allergen extracts were analyzed using the newly developed sandwich enzyme-linked immunosorben"],"journal":["PeerJ"],"pagination":["e17233"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11032652"],"repository":["biostudies-literature"],"pubmed_title":["Novel monoclonal antibodies against house dust mite allergen Der p 21 and their application to analyze allergen extracts."],"pmcid":["PMC11032652"],"pubmed_authors":["Silimavicius L","Zvirbliene A","Rudokas V","Pleckaityte M","Kucinskaite-Kodze I","Sliziene A"],"additional_accession":[]},"is_claimable":false,"name":"Novel monoclonal antibodies against house dust mite allergen Der p 21 and their application to analyze allergen extracts.","description":"<h4>Background</h4>Allergen extracts and recombinant allergens are used in allergy diagnostics and immunotherapy. Since allergen extracts from different manufacturers lack proper standardization regarding their composition, monoclonal antibodies (MAbs) against specific allergen components can be used for their identification and quantification in allergen extracts. This study aimed to generate MAbs against allergen Der p 21 of <i>Dermatophagoides pteronyssinus</i> for the analysis of allergen extracts.<h4>Methods</h4>Recombinant Der p 21 was expressed in <i>E. coli</i> and purified using affinity chromatography. MAbs against Der p 21 were generated using hybridoma technology. House dust mite (HDM) allergen extracts were analyzed using the newly developed sandwich enzyme-linked immunosorben","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-06-01T12:01:06.367Z","creation":"2026-05-18T03:07:35.875Z"},"accession":"S-EPMC11032652","cross_references":{"pubmed":["38646484"],"doi":["10.7717/peerj.17233"]}}