biostudies-literatureUnknown143(14)Bastian L<h4>Abstract</h4>Distinct diagnostic entities within BCR::ABL1-positive acute lymphoblastic leukemia (ALL) are currently defined by the International Consensus Classification of myeloid neoplasms and acute leukemias (ICC): "lymphoid only", with BCR::ABL1 observed exclusively in lymphatic precursors, vs "multilineage", where BCR::ABL1 is also present in other hematopoietic lineages. Here, we analyzed transcriptomes of 327 BCR::ABL1-positive patients with ALL (age, 2-84 years; median, 46 years) and identified 2 main gene expression clusters reproducible across 4 independent patient cohorts. Fluorescence in situ hybridization analysis of fluorescence-activated cell-sorted hematopoietic compartments showed distinct BCR::ABL1 involvement in myeloid cells for these clusters (n = 18/18 vs n = 3/1Blood1391-1398https://www.ebi.ac.uk/biostudies/studies/S-EPMC11033585biostudies-literatureDevelopmental trajectories and cooperating genomic events define molecular subtypes of BCR::ABL1-positive ALL.PMC11033585Gokbuget NWittig MBastian LWolgast NHartmann AMZimmermann JNeumann MBaldus CDHaferlach CBrandl BDas Gupta DChitadze GDenker MFaul CBruggemann MStelljes MSchneller FBendig SSchwartz SMuller FJFiedler WBeder TSteffen BPfeifer HBarz MJIben KHarder LBartsch LRohrandt CWalter WHansen BTCario GfalseDevelopmental trajectories and cooperating genomic events define molecular subtypes of BCR::ABL1-positive ALL.<h4>Abstract</h4>Distinct diagnostic entities within BCR::ABL1-positive acute lymphoblastic leukemia (ALL) are currently defined by the International Consensus Classification of myeloid neoplasms and acute leukemias (ICC): "lymphoid only", with BCR::ABL1 observed exclusively in lymphatic precursors, vs "multilineage", where BCR::ABL1 is also present in other hematopoietic lineages. Here, we analyzed transcriptomes of 327 BCR::ABL1-positive patients with ALL (age, 2-84 years; median, 46 years) and identified 2 main gene expression clusters reproducible across 4 independent patient cohorts. Fluorescence in situ hybridization analysis of fluorescence-activated cell-sorted hematopoietic compartments showed distinct BCR::ABL1 involvement in myeloid cells for these clusters (n = 18/18 vs n = 3/12024-01-01T00:00:00Z2024 Apr2026-06-01T09:20:47.097Z2026-04-08T11:06:20.44ZS-EPMC110335853815391310.1182/blood.2023021752