{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Camino LP"],"funding":["NIEHS NIH HHS","NCI NIH HHS"],"pagination":["3707-3719.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11034902"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["83(20)"],"pubmed_abstract":["R-loops, which consist of a DNA-RNA hybrid and a displaced DNA strand, are known to threaten genome integrity. To counteract this, different mechanisms suppress R-loop accumulation by either preventing the hybridization of RNA with the DNA template (RNA biogenesis factors), unwinding the hybrid (DNA-RNA helicases), or degrading the RNA moiety of the R-loop (type H ribonucleases [RNases H]). Thus far, RNases H are the only nucleases known to cleave DNA-RNA hybrids. Now, we show that the RNase DICER also resolves R-loops. Biochemical analysis reveals that DICER acts by specifically cleaving the RNA within R-loops. Importantly, a DICER RNase mutant impaired in R-loop processing causes a strong accumulation of R-loops in cells. Our results thus not only reveal a function of DICER as an R-loop "],"journal":["Molecular cell"],"pubmed_title":["DICER ribonuclease removes harmful R-loops."],"pmcid":["PMC11034902"],"funding_grant_id":["R35 CA241801","R01 CA168635","R01 ES007061"],"pubmed_authors":["Aguilera A","Dutta A","Gomez-Gonzalez B","Barroso S","Camino LP","Perez-Calero C","Garcia-Rubio M","Katz JN","Sung P"],"additional_accession":[]},"is_claimable":false,"name":"DICER ribonuclease removes harmful R-loops.","description":"R-loops, which consist of a DNA-RNA hybrid and a displaced DNA strand, are known to threaten genome integrity. To counteract this, different mechanisms suppress R-loop accumulation by either preventing the hybridization of RNA with the DNA template (RNA biogenesis factors), unwinding the hybrid (DNA-RNA helicases), or degrading the RNA moiety of the R-loop (type H ribonucleases [RNases H]). Thus far, RNases H are the only nucleases known to cleave DNA-RNA hybrids. Now, we show that the RNase DICER also resolves R-loops. Biochemical analysis reveals that DICER acts by specifically cleaving the RNA within R-loops. Importantly, a DICER RNase mutant impaired in R-loop processing causes a strong accumulation of R-loops in cells. Our results thus not only reveal a function of DICER as an R-loop ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2026-04-19T03:19:41.466Z","creation":"2025-04-04T00:29:42.658Z"},"accession":"S-EPMC11034902","cross_references":{"pubmed":["37827159"],"doi":["10.1016/j.molcel.2023.09.021"]}}