{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Serrano K"],"funding":["DOE | SC | Biological and Environmental Research","DOE | LDRD | Lawrence Berkeley National Laboratory","DOE | SC | Biological and Environmental Research (BER)"],"pagination":["673-688"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11035146"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(4)"],"pubmed_abstract":["The symbiotic interaction of plants with arbuscular mycorrhizal (AM) fungi is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant-fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. Here we used single-nucleus and spatial RNA sequencing to explore both Medicago truncatula and Rhizophagus irregularis transcriptomes in AM symbiosis at cellular and spatial resolution. Integrated, spatially registered single-cell maps revealed infected and uninfected plant root cell types. We observed that cortex cells exhibit distinct transcriptom"],"journal":["Nature plants"],"pubmed_title":["Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis."],"pmcid":["PMC11035146"],"funding_grant_id":["DE-AC02-05CH11231"],"pubmed_authors":["Malmstrom RR","Visel A","Scheller HV","Cole B","O'Malley R","Serrano K","Bezrutczyk M","Dao T","Goudeau D"],"additional_accession":[]},"is_claimable":false,"name":"Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis.","description":"The symbiotic interaction of plants with arbuscular mycorrhizal (AM) fungi is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant-fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. Here we used single-nucleus and spatial RNA sequencing to explore both Medicago truncatula and Rhizophagus irregularis transcriptomes in AM symbiosis at cellular and spatial resolution. Integrated, spatially registered single-cell maps revealed infected and uninfected plant root cell types. We observed that cortex cells exhibit distinct transcriptom","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T19:59:49.673Z","creation":"2026-05-20T03:08:31.486Z"},"accession":"S-EPMC11035146","cross_references":{"pubmed":["38589485"],"doi":["10.1038/s41477-024-01666-3"]}}