{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dar F"],"funding":["NIBIB NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["3413"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11035652"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["The functions of biomolecular condensates are thought to be influenced by their material properties, and these will be determined by the internal organization of molecules within condensates. However, structural characterizations of condensates are challenging, and rarely reported. Here, we deploy a combination of small angle neutron scattering, fluorescence recovery after photobleaching, and coarse-grained molecular dynamics simulations to provide structural descriptions of model condensates that are formed by macromolecules from nucleolar granular components (GCs). We show that these minimal facsimiles of GCs form condensates that are network fluids featuring spatial inhomogeneities across different length scales that reflect the contributions of distinct protein and peptide domains. The network-like inhomogeneous organization is characterized by a coexistence of liquid- and gas-like macromolecular densities that engenders bimodality of internal molecular dynamics. These insights suggest that condensates formed by multivalent proteins share features with network fluids formed by systems such as patchy or hairy colloids."],"journal":["Nature communications"],"pubmed_title":["Biomolecular condensates form spatially inhomogeneous network fluids."],"pmcid":["PMC11035652"],"funding_grant_id":["T32 EB028092","P30 CA021765","R01 GM115634","R01 CA092035","R35 GM131891"],"pubmed_authors":["Choi JM","Kriwacki RW","Mitrea DM","Cohen SR","Dar F","Phillips AH","Leite WC","Pappu RV","Nagy G","Stanley CB"],"additional_accession":[]},"is_claimable":false,"name":"Biomolecular condensates form spatially inhomogeneous network fluids.","description":"The functions of biomolecular condensates are thought to be influenced by their material properties, and these will be determined by the internal organization of molecules within condensates. However, structural characterizations of condensates are challenging, and rarely reported. Here, we deploy a combination of small angle neutron scattering, fluorescence recovery after photobleaching, and coarse-grained molecular dynamics simulations to provide structural descriptions of model condensates that are formed by macromolecules from nucleolar granular components (GCs). We show that these minimal facsimiles of GCs form condensates that are network fluids featuring spatial inhomogeneities across different length scales that reflect the contributions of distinct protein and peptide domains. The network-like inhomogeneous organization is characterized by a coexistence of liquid- and gas-like macromolecular densities that engenders bimodality of internal molecular dynamics. These insights suggest that condensates formed by multivalent proteins share features with network fluids formed by systems such as patchy or hairy colloids.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T19:58:35.274Z","creation":"2026-05-20T03:07:59.207Z"},"accession":"S-EPMC11035652","cross_references":{"pubmed":["38649740"],"doi":["10.1038/s41467-024-47602-z"]}}