biostudies-literatureMorgenstern EDeutsche Forschungsgemeinschafte202302475https://www.ebi.ac.uk/biostudies/studies/S-EPMC11035861biostudies-literatureUnknown7(7)Numerous long non-coding RNAs (lncRNAs) were shown to have a functional impact on cellular processes such as human epidermal homeostasis. However, the mechanism of action for many lncRNAs remains unclear to date. Here, we report that lncRNA LINC00941 regulates keratinocyte differentiation on an epigenetic level through association with the NuRD complex, one of the major chromatin remodelers in cells. We find that LINC00941 interacts with NuRD-associated MTA2 and CHD4 in human primary keratinocytes. LINC00941 perturbation changes MTA2/NuRD occupancy at bivalent chromatin domains in close proximity to transcriptional regulator genes, including the <i>EGR3</i> gene coding for a transcription factor regulating epidermal differentiation. Notably, LINC00941 depletion resulted in reduced NuRD occLife science alliancelncRNA LINC00941 modulates MTA2/NuRD occupancy to suppress premature human epidermal differentiation.PMC11035861SFB 960, project B09Molthof CKretz MSchwartz UHombach SGraf JBruckmann AMorgenstern EfalselncRNA LINC00941 modulates MTA2/NuRD occupancy to suppress premature human epidermal differentiation.Numerous long non-coding RNAs (lncRNAs) were shown to have a functional impact on cellular processes such as human epidermal homeostasis. However, the mechanism of action for many lncRNAs remains unclear to date. Here, we report that lncRNA LINC00941 regulates keratinocyte differentiation on an epigenetic level through association with the NuRD complex, one of the major chromatin remodelers in cells. We find that LINC00941 interacts with NuRD-associated MTA2 and CHD4 in human primary keratinocytes. LINC00941 perturbation changes MTA2/NuRD occupancy at bivalent chromatin domains in close proximity to transcriptional regulator genes, including the <i>EGR3</i> gene coding for a transcription factor regulating epidermal differentiation. Notably, LINC00941 depletion resulted in reduced NuRD occ2024-01-01T00:00:00Z2024 Jul2026-06-01T19:59:28.443Z2026-05-20T03:08:02.713ZS-EPMC110358613864918610.26508/lsa.202302475