biostudies-literatureUnknown27(5)Wegrzyniak OEuropean Foundation for the Study of DiabetesBarndiabetesfondensMagnus Bergvall FoundationSwedish Research CouncilNon-invasive assessment of fibrogenic activity, rather than fibrotic scars, could significantly improve the management of fibrotic diseases and the development of anti-fibrotic drugs. This study explores the potential of an Affibody molecule (Z09591) labeled with the Al(18)F-restrained complexing agent (RESCA) method as a tracer for the non-invasive detection of fibrogenic cells. Z09591 was functionalized with the RESCA chelator for direct labeling with [¹⁸F]AlF. <i>In vivo</i> positron emission tomography/magnetic resonance imaging scans on U-87 tumor-bearing mice exhibited high selectivity of the resulting radiotracer, [¹⁸F]AlF-RESCA-Z09591, for platelet-derived growth factor receptor β (PDGFRβ), with minimal non-specific background uptake. Evaluation in a mouse modiScience109688https://www.ebi.ac.uk/biostudies/studies/S-EPMC11039342biostudies-literatureNon-invasive PET imaging of liver fibrogenesis using a RESCA-conjugated Affibody molecule.PMC11039342Persson JMitran BWegrzyniak OLechi FCheung PNordstrom HKorsgren OFrejd FYEriksson JLofblom JBitzios AEriksson OZhang BfalseNon-invasive PET imaging of liver fibrogenesis using a RESCA-conjugated Affibody molecule.Non-invasive assessment of fibrogenic activity, rather than fibrotic scars, could significantly improve the management of fibrotic diseases and the development of anti-fibrotic drugs. This study explores the potential of an Affibody molecule (Z09591) labeled with the Al(18)F-restrained complexing agent (RESCA) method as a tracer for the non-invasive detection of fibrogenic cells. Z09591 was functionalized with the RESCA chelator for direct labeling with [¹⁸F]AlF. <i>In vivo</i> positron emission tomography/magnetic resonance imaging scans on U-87 tumor-bearing mice exhibited high selectivity of the resulting radiotracer, [¹⁸F]AlF-RESCA-Z09591, for platelet-derived growth factor receptor β (PDGFRβ), with minimal non-specific background uptake. Evaluation in a mouse mod2024-01-01T00:00:00Z2024 May2026-06-02T01:43:44.811Z2025-04-06T03:15:49.022ZS-EPMC110393423866040510.1016/j.isci.2024.109688