biostudies-literatureJuaiti MNational Natural Science Foundation of ChinaHunan Provincial Natural Science Foundatione29587https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040037biostudies-literatureUnknown10(8)<h4>Background</h4>Pulmonary arterial hypertension (PAH) represents a substantial global risk to human health. This study aims to identify diagnostic biomarkers for PAH and assess their association with the immune microenvironment through the utilization of sophisticated bioinformatics techniques.<h4>Methods</h4>Based on two microarray datasets, differentially expressed genes (DEGs) were detected, and hub genes underwent a sequence of machine learning analyses. After pathways associated with PAH were assessed by gene enrichment analysis, the identified genes were validated using external datasets and confirmed in a monocrotaline (MCT)-induced rat model. In addition, three algorithms were employed to estimate the proportions of various immune cell types, and the link between hub genes and iHeliyonIntegrated bioinformatics analysis and experimental animal models identify a robust biomarker and its correlation with the immune microenvironment in pulmonary arterial hypertension.PMC1104003782070055Juaiti MFeng YZha LYu ZTang YLiang BfalseIntegrated bioinformatics analysis and experimental animal models identify a robust biomarker and its correlation with the immune microenvironment in pulmonary arterial hypertension.<h4>Background</h4>Pulmonary arterial hypertension (PAH) represents a substantial global risk to human health. This study aims to identify diagnostic biomarkers for PAH and assess their association with the immune microenvironment through the utilization of sophisticated bioinformatics techniques.<h4>Methods</h4>Based on two microarray datasets, differentially expressed genes (DEGs) were detected, and hub genes underwent a sequence of machine learning analyses. After pathways associated with PAH were assessed by gene enrichment analysis, the identified genes were validated using external datasets and confirmed in a monocrotaline (MCT)-induced rat model. In addition, three algorithms were employed to estimate the proportions of various immune cell types, and the link between hub genes and i2024-01-01T00:00:00Z2024 Apr2026-07-01T03:22:00.959Z2026-07-01T03:12:29.48ZS-EPMC110400373866027110.1016/j.heliyon.2024.e29587