biostudies-literatureJoo JHKun-hee Lee Seoul National University Hospital Child Cancer & Rare Disease Project, Republic of KoreaNational Research Foundation of KoreaNational Institutes of HealthNIGMS NIH HHSNIH HHS3794-3809https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040155biostudies-literatureUnknown52(7)Meiotic recombination is initiated by programmed double-strand breaks (DSBs). Studies in Saccharomyces cerevisiae have shown that, following rapid resection to generate 3' single-stranded DNA (ssDNA) tails, one DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas the other end remains associated with its sister. Then, after regulated differentiation into crossover- and noncrossover-fated types, the second DSB end participates in the reaction by strand annealing with the extended first end, along both pathways. This second-end capture is dependent on Rad52, presumably via its known capacity to anneal two ssDNAs. Here, using physical analysis of DNA recombination, we demonstrate that this process is dependent on direct interaction of Rad52 with the ssDNA bindNucleic acids researchRPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.PMC11040155R35 GM1363222021R1I1A1A01059573RS-2023-0020819122B-001-05002022M3A9J40794682022R1I1A1A01055532R35-GM-1363222018R1A5A1025077Joo JHChoi EHKim KPLee MSHigashide MTYoon SShinohara AKleckner NKang HAHong SfalseRPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.Meiotic recombination is initiated by programmed double-strand breaks (DSBs). Studies in Saccharomyces cerevisiae have shown that, following rapid resection to generate 3' single-stranded DNA (ssDNA) tails, one DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas the other end remains associated with its sister. Then, after regulated differentiation into crossover- and noncrossover-fated types, the second DSB end participates in the reaction by strand annealing with the extended first end, along both pathways. This second-end capture is dependent on Rad52, presumably via its known capacity to anneal two ssDNAs. Here, using physical analysis of DNA recombination, we demonstrate that this process is dependent on direct interaction of Rad52 with the ssDNA bind2024-01-01T00:00:00Z2024 Apr2025-04-22T20:43:05.393Z2025-04-06T03:14:41.637ZS-EPMC110401553834033910.1093/nar/gkae083