{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu T"],"funding":["Medical and Industry Joint Innovation Programs","National Natural Science Foundation of China","Innovation Program","Affiliated Hospital of Dalian Medical University and DICP","DICP, CAS","National Key Research and Development Program of China"],"pagination":["e2306659"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040340"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(16)"],"pubmed_abstract":["High-coverage mass spectrometry analysis of single-cell metabolomics remains challenging due to the extremely low abundance and wide polarity of metabolites and ultra-small volume in single cells. Herein, a novel concentric hybrid ionization source, nanoelectrospray ionization-atmospheric pressure chemical ionization (nanoESI-APCI), is ingeniously designed to detect polar and nonpolar metabolites simultaneously in single cells. The source is constructed by inserting a pulled glass capillary coaxially into a glass tube that acts as a dielectric barrier layer. Benefitting from the integrated advantages of nanoESI and APCI, its limit of detection is improved by one order of magnitude to 10 pg mL<sup>-1</sup>. After the operational parameter optimization, 254 metabolites detected in nanoESI-AP"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Concentric Hybrid Nanoelectrospray Ionization-Atmospheric Pressure Chemical Ionization Source for High-Coverage Mass Spectrometry Analysis of Single-Cell Metabolomics."],"pmcid":["PMC11040340"],"funding_grant_id":["DMU-1&DICP UN202203","22274156","DICP I202141","22204163","2022YFC3401200","21934006","DMU-2&DICP UN202302","22074144"],"pubmed_authors":["Li H","Tan G","Hu X","Dou P","Feng D","Chen C","Xu G","Shi X","Hu C","Mu H","Zhang Z","Wang T","Xu T","Luo Y","Pu S"],"additional_accession":[]},"is_claimable":false,"name":"Concentric Hybrid Nanoelectrospray Ionization-Atmospheric Pressure Chemical Ionization Source for High-Coverage Mass Spectrometry Analysis of Single-Cell Metabolomics.","description":"High-coverage mass spectrometry analysis of single-cell metabolomics remains challenging due to the extremely low abundance and wide polarity of metabolites and ultra-small volume in single cells. Herein, a novel concentric hybrid ionization source, nanoelectrospray ionization-atmospheric pressure chemical ionization (nanoESI-APCI), is ingeniously designed to detect polar and nonpolar metabolites simultaneously in single cells. The source is constructed by inserting a pulled glass capillary coaxially into a glass tube that acts as a dielectric barrier layer. Benefitting from the integrated advantages of nanoESI and APCI, its limit of detection is improved by one order of magnitude to 10 pg mL<sup>-1</sup>. After the operational parameter optimization, 254 metabolites detected in nanoESI-AP","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2025-04-22T20:44:37.323Z","creation":"2025-04-06T03:15:17.898Z"},"accession":"S-EPMC11040340","cross_references":{"pubmed":["38359005"],"doi":["10.1002/advs.202306659"]}}