{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liao Y"],"funding":["Société d’Accélération du Transfert de Technologies","China Scholarship Council","Fondation ARC pour la Recherche sur le Cancer","Agence Nationale de la Recherche","French Infrastructure for Integrated Structural Biology","University of Geneva","Programme Fédérateur Aviesan","Sanofi iAward Europe","Institut de génétique et de biologie moléculaire et cellulaire","IMC-Bio","Australian Research Council","Institut National du Cancer","Schweizerischen Nationalfonds","Société d'Accélération du Transfert de Technologies","Instruct-ERIC","iNext-Discovery","NANOTUMOR","Centre National de la Recherche Scientifique","Fondation pour la Recherche Médicale","Plan Cancer"],"pagination":["e202310006"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040503"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["223(7)"],"pubmed_abstract":["Assembly of macromolecular complexes at correct cellular sites is crucial for cell function. Nuclear pore complexes (NPCs) are large cylindrical assemblies with eightfold rotational symmetry, built through hierarchical binding of nucleoporins (Nups) forming distinct subcomplexes. Here, we uncover a role of ubiquitin-associated protein 2-like (UBAP2L) in the assembly and stability of properly organized and functional NPCs at the intact nuclear envelope (NE) in human cells. UBAP2L localizes to the nuclear pores and facilitates the formation of the Y-complex, an essential scaffold component of the NPC, and its localization to the NE. UBAP2L promotes the interaction of the Y-complex with POM121 and Nup153, the critical upstream factors in a well-defined sequential order of Nups assembly onto NE during interphase. Timely localization of the cytoplasmic Nup transport factor fragile X-related protein 1 (FXR1) to the NE and its interaction with the Y-complex are likewise dependent on UBAP2L. Thus, this NPC biogenesis mechanism integrates the cytoplasmic and the nuclear NPC assembly signals and ensures efficient nuclear transport, adaptation to nutrient stress, and cellular proliferative capacity, highlighting the importance of NPC homeostasis at the intact NE."],"journal":["The Journal of cell biology"],"pubmed_title":["UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope."],"pmcid":["PMC11040503"],"funding_grant_id":["NICE4Nups","AAPG2022","PLBIO 2022-082","ANR-10-LABX-0030-INRT","ANR-10-IDEX-0002-02","310030_204267","ANR-10-INSB-05-01"],"pubmed_authors":["Riveline D","Liao Y","Cirillo L","Klaholz BP","Sumara I","Liu X","Lu L","Agote-Aran A","Zhang Z","Guerber L","Qu M","Andronov L","Kleiss C","Pangou E","Ran L","Lin J","Gotta M","Schmucker S"],"additional_accession":[]},"is_claimable":false,"name":"UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope.","description":"Assembly of macromolecular complexes at correct cellular sites is crucial for cell function. Nuclear pore complexes (NPCs) are large cylindrical assemblies with eightfold rotational symmetry, built through hierarchical binding of nucleoporins (Nups) forming distinct subcomplexes. Here, we uncover a role of ubiquitin-associated protein 2-like (UBAP2L) in the assembly and stability of properly organized and functional NPCs at the intact nuclear envelope (NE) in human cells. UBAP2L localizes to the nuclear pores and facilitates the formation of the Y-complex, an essential scaffold component of the NPC, and its localization to the NE. UBAP2L promotes the interaction of the Y-complex with POM121 and Nup153, the critical upstream factors in a well-defined sequential order of Nups assembly onto NE during interphase. Timely localization of the cytoplasmic Nup transport factor fragile X-related protein 1 (FXR1) to the NE and its interaction with the Y-complex are likewise dependent on UBAP2L. Thus, this NPC biogenesis mechanism integrates the cytoplasmic and the nuclear NPC assembly signals and ensures efficient nuclear transport, adaptation to nutrient stress, and cellular proliferative capacity, highlighting the importance of NPC homeostasis at the intact NE.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-01T23:56:09.936Z","creation":"2026-05-24T03:07:31.533Z"},"accession":"S-EPMC11040503","cross_references":{"pubmed":["38652117"],"doi":["10.1083/jcb.202310006"]}}