biostudies-literatureCastanheira EJH2020 LEIT NanotechnologiesFunda??o para a Ci?ncia e a Tecnologia18386-18399https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040583biostudies-literatureUnknown16(15)Cryogels exhibit unique shape memory with full recovery and structural stability features after multiple injections. These constructs also possess enhanced cell permeability and nutrient diffusion when compared to typical bulk hydrogels. Volumetric processing of cryogels functionalized with nanosized units has potential to widen their biomedical applications, however this has remained challenging and relatively underexplored. In this study, we report a novel methodology that combines suspension 3D printing with directional freezing for the fabrication of nanocomposite cryogels with configurable anisotropy. When compared to conventional bulk or freeze-dried hydrogels, nanocomposite cryogel formulations exhibit excellent shape recovery (>95%) and higher pore connectivity. Suspension printingACS applied materials & interfacesIn-Bath 3D Printing of Anisotropic Shape-Memory Cryogels Functionalized with Bone-Bioactive Nanoparticles.PMC11040583SFRH/BD/144880/2019UIDP/50011/2020 (DOI 10.54499/UIDP/50011/2020)PTDC/QUIQOR/30771/2017 POCI-01-0145-FEDER-30772020.06767.BDDOI 10.54499/2022.02106.CEECIND/CP1720/CT0028953169DOI 10.54499/CEECIND/01363/2018/CP1559/CT0022LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020)UIDB/50011/2020 (DOI 10.54499/UIDB/50011/2020)Mano JFCorreia TRCastanheira EJGaspar VMMonteiro LPGRodrigues JMMfalseIn-Bath 3D Printing of Anisotropic Shape-Memory Cryogels Functionalized with Bone-Bioactive Nanoparticles.Cryogels exhibit unique shape memory with full recovery and structural stability features after multiple injections. These constructs also possess enhanced cell permeability and nutrient diffusion when compared to typical bulk hydrogels. Volumetric processing of cryogels functionalized with nanosized units has potential to widen their biomedical applications, however this has remained challenging and relatively underexplored. In this study, we report a novel methodology that combines suspension 3D printing with directional freezing for the fabrication of nanocomposite cryogels with configurable anisotropy. When compared to conventional bulk or freeze-dried hydrogels, nanocomposite cryogel formulations exhibit excellent shape recovery (>95%) and higher pore connectivity. Suspension printing2024-01-01T00:00:00Z2024 Apr2025-04-22T20:44:10.167Z2025-04-06T03:15:06.964ZS-EPMC110405833859124310.1021/acsami.3c18290