biostudies-literatureBo CExcellent Scientific Research and Innovation Team of University in Anhui ProvinceProject of Natural Science Research of Universities in Anhui Province, ChinaNational Natural Science Foundation of ChinaKey Research and Development Program of Shandong Province, China398https://www.ebi.ac.uk/biostudies/studies/S-EPMC11040748biostudies-literatureUnknown25(1)Pinellia ternata (Thunb.) Briet., a valuable herb native to China, is susceptible to the "sprout tumble" phenomenon because of high temperatures, resulting in a significant yield reduction. However, the molecular regulatory mechanisms underlying the response of P. ternata to heat stress are not well understood. In this study, we integrated transcriptome and miRNAome sequencing to identify heat-response genes, microRNAs (miRNAs), and key miRNA-target pairs in P. ternata that differed between heat-stress and room-temperature conditions. Transcriptome analysis revealed extensive reprogramming of 4,960 genes across various categories, predominantly associated with cellular and metabolic processes, responses to stimuli, biological regulation, cell parts, organelles, membranes, and catalytic andBMC genomicsIntegrated analysis of transcriptome and miRNAome reveals the heat stress response of Pinellia ternata seedlings.PMC1104074882373993822740482022AH0100292019GNC1061132023AH050352Liu XXue TLiu MZhu YWang DYou QXue JBo CDuan YfalseIntegrated analysis of transcriptome and miRNAome reveals the heat stress response of Pinellia ternata seedlings.Pinellia ternata (Thunb.) Briet., a valuable herb native to China, is susceptible to the "sprout tumble" phenomenon because of high temperatures, resulting in a significant yield reduction. However, the molecular regulatory mechanisms underlying the response of P. ternata to heat stress are not well understood. In this study, we integrated transcriptome and miRNAome sequencing to identify heat-response genes, microRNAs (miRNAs), and key miRNA-target pairs in P. ternata that differed between heat-stress and room-temperature conditions. Transcriptome analysis revealed extensive reprogramming of 4,960 genes across various categories, predominantly associated with cellular and metabolic processes, responses to stimuli, biological regulation, cell parts, organelles, membranes, and catalytic and2024-01-01T00:00:00Z2024 Apr2026-06-01T09:17:22.32Z2026-04-08T11:05:53.577ZS-EPMC110407483865415010.1186/s12864-024-10318-x