{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Z"],"funding":["Ministry of Science and Technology of the People&apos;s Republic of China","National Natural Science Foundation of China"],"pagination":["109676"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11043890"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(5)"],"pubmed_abstract":["Growing evidences indicate that RNA-binding proteins (RBPs) play critical roles in regulating the RNA splicing, polyadenylation, stability, localization, translation, and turnover. Abnormal expression of RBPs can promote tumorigenesis. Here, we performed a CRISPR screen using an RBP pooled CRISPR knockout library and identified 27 potential RBPs with role in supporting colorectal cancer (CRC) survival. We found that the deletion/depletion of INTS3 triggered apoptosis in CRC. The <i>in vitro</i> experiments and RNA sequencing revealed that INTS3 destabilized pro-apoptotic gene transcripts and contributed to the survival of CRC cells. INTS3 loss delayed CRC cells growth <i>in vivo</i>. Furthermore, delivery of DOTAP/cholesterol-mshINTS3 nanoparticles inhibited CRC tumor growth. Collectively,"],"journal":["iScience"],"pubmed_title":["CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer."],"pmcid":["PMC11043890"],"funding_grant_id":["32200652","82173176","2020YFA0803500"],"pubmed_authors":["Li P","Zeng X","Liu S","Zhang L","He Q","Zhu P","Yang Y","Guo J","Chen Z","Zhao Q","Zhai J","Wang X","Zhang C","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer.","description":"Growing evidences indicate that RNA-binding proteins (RBPs) play critical roles in regulating the RNA splicing, polyadenylation, stability, localization, translation, and turnover. Abnormal expression of RBPs can promote tumorigenesis. Here, we performed a CRISPR screen using an RBP pooled CRISPR knockout library and identified 27 potential RBPs with role in supporting colorectal cancer (CRC) survival. We found that the deletion/depletion of INTS3 triggered apoptosis in CRC. The <i>in vitro</i> experiments and RNA sequencing revealed that INTS3 destabilized pro-apoptotic gene transcripts and contributed to the survival of CRC cells. INTS3 loss delayed CRC cells growth <i>in vivo</i>. Furthermore, delivery of DOTAP/cholesterol-mshINTS3 nanoparticles inhibited CRC tumor growth. Collectively,","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-03T03:57:26.114Z","creation":"2026-04-24T03:09:31.042Z"},"accession":"S-EPMC11043890","cross_references":{"pubmed":["38665208"],"doi":["10.1016/j.isci.2024.109676"]}}