{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Williams BL"],"funding":["NEI NIH HHS"],"pagination":["34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11044837"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["65(4)"],"pubmed_abstract":["<h4>Purpose</h4>The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process.<h4>Methods</h4>A custom-made sandwich ELISA assay (SCTM ELISA) for detection of the HtrA1 protein was designed and compared with three commercial assays (R&D Systems, MyBiosource 1 and MyBiosource 2) using 65 serum samples. Concentrations of HtrA1 were thereafter determined in serum and vitreous samples collected from 248 individuals and 145 human donor eyes, respectively.<h4>Results</h4>The SCTM ELISA demonstrated high specificity"],"journal":["Investigative ophthalmology & visual science"],"pubmed_title":["Levels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus."],"pmcid":["PMC11044837"],"funding_grant_id":["R24 EY017404"],"pubmed_authors":["Liu J","Hageman JL","Hageman GS","Williams BL","Seager NA","Hubbard WC","Mohler J","Richards BT","Thomas J","Zouache MA","Pappas CM","Anstadt RA"],"additional_accession":[]},"is_claimable":false,"name":"Levels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus.","description":"<h4>Purpose</h4>The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process.<h4>Methods</h4>A custom-made sandwich ELISA assay (SCTM ELISA) for detection of the HtrA1 protein was designed and compared with three commercial assays (R&D Systems, MyBiosource 1 and MyBiosource 2) using 65 serum samples. Concentrations of HtrA1 were thereafter determined in serum and vitreous samples collected from 248 individuals and 145 human donor eyes, respectively.<h4>Results</h4>The SCTM ELISA demonstrated high specificity","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-03T03:55:14.315Z","creation":"2025-08-14T03:05:49.379Z"},"accession":"S-EPMC11044837","cross_references":{"pubmed":["38648039"],"doi":["10.1167/iovs.65.4.34"]}}