biostudies-literatureWilliams BLNEI NIH HHS34https://www.ebi.ac.uk/biostudies/studies/S-EPMC11044837biostudies-literatureUnknown65(4)<h4>Purpose</h4>The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process.<h4>Methods</h4>A custom-made sandwich ELISA assay (SCTM ELISA) for detection of the HtrA1 protein was designed and compared with three commercial assays (R&D Systems, MyBiosource 1 and MyBiosource 2) using 65 serum samples. Concentrations of HtrA1 were thereafter determined in serum and vitreous samples collected from 248 individuals and 145 human donor eyes, respectively.<h4>Results</h4>The SCTM ELISA demonstrated high specificityInvestigative ophthalmology & visual scienceLevels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus.PMC11044837R24 EY017404Liu JHageman JLHageman GSWilliams BLSeager NAHubbard WCMohler JRichards BTThomas JZouache MAPappas CMAnstadt RAfalseLevels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus.<h4>Purpose</h4>The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process.<h4>Methods</h4>A custom-made sandwich ELISA assay (SCTM ELISA) for detection of the HtrA1 protein was designed and compared with three commercial assays (R&D Systems, MyBiosource 1 and MyBiosource 2) using 65 serum samples. Concentrations of HtrA1 were thereafter determined in serum and vitreous samples collected from 248 individuals and 145 human donor eyes, respectively.<h4>Results</h4>The SCTM ELISA demonstrated high specificity2024-01-01T00:00:00Z2024 Apr2026-06-03T03:55:14.315Z2025-08-14T03:05:49.379ZS-EPMC110448373864803910.1167/iovs.65.4.34