biostudies-literatureBilinsky LMNational Institute of Environmental Health SciencesNIEHS NIH HHSU.S. Food and Drug Administration100299https://www.ebi.ac.uk/biostudies/studies/S-EPMC11044893biostudies-literatureUnknown29This paper presents an ordinary differential equation (ODE) model of endogenous H₂O₂ production and elimination in hepatocytes that is unique, at the time of writing, in its ability to accurately compute intracellular H₂O₂ concentration during incidents of oxidative stress and in its usefulness for constructing PBPK/PD models for ROS-generating xenobiotics. Versions of the model are presented for rat hepatocytes <i>in vitro</i> and mouse liver <i>in vivo</i>. A generic method is given for using the model to create PBPK/PD models which predict intracellular H₂O₂ concentration and oxidative-stress-induced hepatocyte death; these are identifiable from <i>in vitro</i> data sets reporting cell mortality following xenobiotic exposComputational toxicology (Amsterdam, Netherlands)A Computational Model of Endogenous Hydrogen Peroxide Metabolism in Hepatocytes, Featuring a Critical Role for GSH.PMC1104489312013Y01 S12013001FDA IAG #224-17-0502Bilinsky LMfalseA Computational Model of Endogenous Hydrogen Peroxide Metabolism in Hepatocytes, Featuring a Critical Role for GSH.This paper presents an ordinary differential equation (ODE) model of endogenous H₂O₂ production and elimination in hepatocytes that is unique, at the time of writing, in its ability to accurately compute intracellular H₂O₂ concentration during incidents of oxidative stress and in its usefulness for constructing PBPK/PD models for ROS-generating xenobiotics. Versions of the model are presented for rat hepatocytes <i>in vitro</i> and mouse liver <i>in vivo</i>. A generic method is given for using the model to create PBPK/PD models which predict intracellular H₂O₂ concentration and oxidative-stress-induced hepatocyte death; these are identifiable from <i>in vitro</i> data sets reporting cell mortality following xenobiotic expos2024-01-01T00:00:00Z2024 Mar2025-04-04T02:46:45.219Z2025-04-04T02:46:45.219ZS-EPMC110448933868212710.1016/j.comtox.2024.100299