biostudies-literaturePacheco-Moreno AJohn Innes FoundationBiotechnology and Biological Sciences Research Councile3002232https://www.ebi.ac.uk/biostudies/studies/S-EPMC11045101biostudies-literatureUnknown22(4)Plant-associated microbes play vital roles in promoting plant growth and health, with plants secreting root exudates into the rhizosphere to attract beneficial microbes. Exudate composition defines the nature of microbial recruitment, with different plant species attracting distinct microbiota to enable optimal adaptation to the soil environment. To more closely examine the relationship between plant genotype and microbial recruitment, we analysed the rhizosphere microbiomes of landrace (Chevallier) and modern (NFC Tipple) barley (Hordeum vulgare) cultivars. Distinct differences were observed between the plant-associated microbiomes of the 2 cultivars, with the plant-growth promoting rhizobacterial genus Pseudomonas substantially more abundant in the Tipple rhizosphere. Striking differencePLoS biologyThe genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion.PMC11045101BB/T001801/1BBS/E/J/000PR9797BB/K02003X/1BB/M011216/1Ramachandran VMalone JGRidout CChandra GNicholson PBrett PBrown JKMPacheco-Moreno AThornton OPoole PDavies JDeVos SBollmann-Giolai AfalseThe genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion.Plant-associated microbes play vital roles in promoting plant growth and health, with plants secreting root exudates into the rhizosphere to attract beneficial microbes. Exudate composition defines the nature of microbial recruitment, with different plant species attracting distinct microbiota to enable optimal adaptation to the soil environment. To more closely examine the relationship between plant genotype and microbial recruitment, we analysed the rhizosphere microbiomes of landrace (Chevallier) and modern (NFC Tipple) barley (Hordeum vulgare) cultivars. Distinct differences were observed between the plant-associated microbiomes of the 2 cultivars, with the plant-growth promoting rhizobacterial genus Pseudomonas substantially more abundant in the Tipple rhizosphere. Striking difference2024-01-01T00:00:00Z2024 Apr2026-06-02T02:15:50.129Z2026-04-13T03:12:19.545ZS-EPMC110451013866264410.1371/journal.pbio.3002232