{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma S"],"funding":["Fondation pour la Recherche Médicale (Foundation for Medical Research in France)","National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["501"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11045811"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(1)"],"pubmed_abstract":["G protein-coupled receptors naturally oscillate between inactive and active states, often resulting in receptor constitutive activity with important physiological consequences. Among the class C G protein-coupled receptors that typically sense amino-acids and their derivatives, the calcium sensing receptor (CaSR) tightly controls blood calcium levels. Its constitutive activity has not yet been studied. Here, we demonstrate the importance of the inter-subunit disulfide bridges in maintaining the inactive state of CaSR, resulting in undetectable constitutive activity, unlike the other class C receptors. Deletion of these disulfide bridges results in strong constitutive activity that is abolished by mutations preventing amino acid binding. It shows that this inter-subunit disulfide link is ne"],"journal":["Communications biology"],"pubmed_title":["Absence of calcium-sensing receptor basal activity due to inter-subunit disulfide bridges."],"pmcid":["PMC11045811"],"funding_grant_id":["31070737","31371423","EQU202303016470","32330049"],"pubmed_authors":["Yin X","Liu J","Ma S","Yi P","Pin JP","Rondard P"],"additional_accession":[]},"is_claimable":false,"name":"Absence of calcium-sensing receptor basal activity due to inter-subunit disulfide bridges.","description":"G protein-coupled receptors naturally oscillate between inactive and active states, often resulting in receptor constitutive activity with important physiological consequences. Among the class C G protein-coupled receptors that typically sense amino-acids and their derivatives, the calcium sensing receptor (CaSR) tightly controls blood calcium levels. Its constitutive activity has not yet been studied. Here, we demonstrate the importance of the inter-subunit disulfide bridges in maintaining the inactive state of CaSR, resulting in undetectable constitutive activity, unlike the other class C receptors. Deletion of these disulfide bridges results in strong constitutive activity that is abolished by mutations preventing amino acid binding. It shows that this inter-subunit disulfide link is ne","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-02T08:40:01.491Z","creation":"2026-05-25T03:06:57.481Z"},"accession":"S-EPMC11045811","cross_references":{"pubmed":["38664468"],"doi":["10.1038/s42003-024-06189-3"]}}