{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(4)"],"submitter":["Nagase M"],"funding":["AMED"],"pubmed_abstract":["Intracellular signaling plays essential roles in various cell types. In the central nervous system, signaling cascades are strictly regulated in a spatiotemporally specific manner to govern brain function; for example, presynaptic cyclic adenosine monophosphate (cAMP) can enhance the probability of neurotransmitter release. In the last decade, channelrhodopsin-2 has been engineered for subcellular targeting using localization tags, but optogenetic tools for intracellular signaling are not well developed. Therefore, we engineered a selective presynaptic fusion tag for photoactivated adenylyl cyclase (bPAC-Syn1a) and found its high localization at presynaptic terminals. Furthermore, an all-optical electrophysiological method revealed rapid and robust short-term potentiation by bPAC-Syn1a at brain stem-amygdala synapses in acute brain slices. Additionally, bPAC-Syn1a modulated mouse immobility behavior. These results indicate that bPAC-Syn1a can manipulate presynaptic cAMP signaling in vitro and in vivo. The all-optical manipulation technique developed in this study can help further elucidate the dynamic regulation of various cellular functions."],"journal":["Cell reports methods"],"pagination":["100740"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11045876"],"repository":["biostudies-literature"],"pubmed_title":["All-optical presynaptic plasticity induction by photoactivated adenylyl cyclase targeted to axon terminals."],"pmcid":["PMC11045876"],"pubmed_authors":["Hirano T","Watabe AM","Nagashima T","Arima-Yoshida F","Hiyoshi K","Morishima M","Hamada S","Nagase M","Ohtsuka T","Tohyama S"],"additional_accession":[]},"is_claimable":false,"name":"All-optical presynaptic plasticity induction by photoactivated adenylyl cyclase targeted to axon terminals.","description":"Intracellular signaling plays essential roles in various cell types. In the central nervous system, signaling cascades are strictly regulated in a spatiotemporally specific manner to govern brain function; for example, presynaptic cyclic adenosine monophosphate (cAMP) can enhance the probability of neurotransmitter release. In the last decade, channelrhodopsin-2 has been engineered for subcellular targeting using localization tags, but optogenetic tools for intracellular signaling are not well developed. Therefore, we engineered a selective presynaptic fusion tag for photoactivated adenylyl cyclase (bPAC-Syn1a) and found its high localization at presynaptic terminals. Furthermore, an all-optical electrophysiological method revealed rapid and robust short-term potentiation by bPAC-Syn1a at brain stem-amygdala synapses in acute brain slices. Additionally, bPAC-Syn1a modulated mouse immobility behavior. These results indicate that bPAC-Syn1a can manipulate presynaptic cAMP signaling in vitro and in vivo. The all-optical manipulation technique developed in this study can help further elucidate the dynamic regulation of various cellular functions.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T21:18:05.995Z","creation":"2026-05-21T03:08:04.283Z"},"accession":"S-EPMC11045876","cross_references":{"pubmed":["38521059"],"doi":["10.1016/j.crmeth.2024.100740"]}}