{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(4)"],"submitter":["Kohler AR"],"funding":["Baden-Wurttemberg Stiftung gGmbH"],"pubmed_abstract":["Dynamic changes in the epigenome at defined genomic loci play crucial roles during cellular differentiation and disease development. Here, we developed dual-color bimolecular anchor detector (BiAD) sensors for high-sensitivity readout of locus-specific epigenome modifications by fluorescence microscopy. Our BiAD sensors comprise an sgRNA/dCas9 complex as anchor and double chromatin reader domains as detector modules, both fused to complementary parts of a split IFP2.0 fluorophore, enabling its reconstitution upon binding of both parts in close proximity. In addition, a YPet fluorophore is recruited to the sgRNA to mark the genomic locus of interest. With these dual-color BiAD sensors, we detected H3K9me2/3 and DNA methylation and their dynamic changes upon RNAi or inhibitor treatment with high sensitivity at endogenous genomic regions. Furthermore, we showcased locus-specific H3K36me2/3 readout as well as H3K27me3 and H3K9me2/3 enrichment on the inactive X chromosome, highlighting the broad applicability of our dual-color BiAD sensors for single-cell epigenome studies."],"journal":["Cell reports methods"],"pagination":["100739"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11045877"],"repository":["biostudies-literature"],"pubmed_title":["Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells."],"pmcid":["PMC11045877"],"pubmed_authors":["Bernhardt S","Haußermann L","Jeltsch A","Haußer J","Olayioye MA","Lungu C","Kohler AR","Brenner LM","Bashtrykov P","Harsch A"],"additional_accession":[]},"is_claimable":false,"name":"Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells.","description":"Dynamic changes in the epigenome at defined genomic loci play crucial roles during cellular differentiation and disease development. Here, we developed dual-color bimolecular anchor detector (BiAD) sensors for high-sensitivity readout of locus-specific epigenome modifications by fluorescence microscopy. Our BiAD sensors comprise an sgRNA/dCas9 complex as anchor and double chromatin reader domains as detector modules, both fused to complementary parts of a split IFP2.0 fluorophore, enabling its reconstitution upon binding of both parts in close proximity. In addition, a YPet fluorophore is recruited to the sgRNA to mark the genomic locus of interest. With these dual-color BiAD sensors, we detected H3K9me2/3 and DNA methylation and their dynamic changes upon RNAi or inhibitor treatment with high sensitivity at endogenous genomic regions. Furthermore, we showcased locus-specific H3K36me2/3 readout as well as H3K27me3 and H3K9me2/3 enrichment on the inactive X chromosome, highlighting the broad applicability of our dual-color BiAD sensors for single-cell epigenome studies.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-02T02:13:25.527Z","creation":"2026-04-13T03:12:45.2Z"},"accession":"S-EPMC11045877","cross_references":{"pubmed":["38554702"],"doi":["10.1016/j.crmeth.2024.100739"]}}