{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao Y"],"funding":["National Natural Science Foundation of China","Youth Program of Natural Science Foundation of Anhui Province","Youth Fund of National Natural Science Foundation Projects"],"pagination":["11769343241249017"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11047246"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20"],"pubmed_abstract":["<h4>Background</h4>Intestinal metaplasia (IM) of gastric epithelium has traditionally been regarded as an irreversible stage in the process of the Correa cascade. Exploring the potential molecular mechanism of IM is significant for effective gastric cancer prevention.<h4>Methods</h4>The GSE78523 dataset, obtained from the Gene Expression Omnibus (GEO) database, was analyzed using RStudio software to identify the differently expressed genes (DEGs) between IM tissues and normal gastric epithelial tissues. Subsequently, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, Gene Set Enrichment Analysis (GESA), and protein-protein interaction (PPI) analysis were used to find potential genes. Additionally, the screened genes were analyzed for clinical, immunological, and genetic correlation aspects using single gene clinical correlation analysis (UALCAN), Tumor-Immune System Interactions Database (TISIDB), and validated through western blot experiments.<h4>Results</h4>Enrichment analysis showed that the lipid metabolic pathway was significantly associated with IM tissues and the apolipoprotein B (<i>APOB</i>) gene was identified in the subsequent analysis. Experiment results and correlation analysis showed that the expression of <i>APOB</i> was higher in IM tissues than in normal tissues. This elevated expression of <i>APOB</i> was also found to be associated with the expression levels of hepatocyte nuclear factor 4A (<i>HNF4A</i>) gene. <i>HNF4A</i> was also found to be associated with immune cell infiltration to gastric cancer and was linked to the prognosis of gastric cancer patients. Moreover, <i>HNF4A</i> was also highly expressed in both IM tissues and gastric cancer cells.<h4>Conclusion</h4>Our findings indicate that <i>HNF4A</i> regulates the microenvironment of lipid metabolism in IM tissues by targeting <i>APOB</i>. Higher expression of <i>HNF4A</i> tends to lead to a worse prognosis in gastric cancer patients implying it may serve as a predictive indicator for the progression from IM to gastric cancer."],"journal":["Evolutionary bioinformatics online"],"pubmed_title":["HNF4A-Bridging the Gap Between Intestinal Metaplasia and Gastric Cancer."],"pmcid":["PMC11047246"],"funding_grant_id":["No. 61976007","No. 2008085QH415","No. 82103040"],"pubmed_authors":["Li Y","Sun F","Zhao Y","Xu J","Tang H"],"additional_accession":[]},"is_claimable":false,"name":"HNF4A-Bridging the Gap Between Intestinal Metaplasia and Gastric Cancer.","description":"<h4>Background</h4>Intestinal metaplasia (IM) of gastric epithelium has traditionally been regarded as an irreversible stage in the process of the Correa cascade. Exploring the potential molecular mechanism of IM is significant for effective gastric cancer prevention.<h4>Methods</h4>The GSE78523 dataset, obtained from the Gene Expression Omnibus (GEO) database, was analyzed using RStudio software to identify the differently expressed genes (DEGs) between IM tissues and normal gastric epithelial tissues. Subsequently, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, Gene Set Enrichment Analysis (GESA), and protein-protein interaction (PPI) analysis were used to find potential genes. Additionally, the screened genes were analyzed for clinical, immunological, and genetic correlation aspects using single gene clinical correlation analysis (UALCAN), Tumor-Immune System Interactions Database (TISIDB), and validated through western blot experiments.<h4>Results</h4>Enrichment analysis showed that the lipid metabolic pathway was significantly associated with IM tissues and the apolipoprotein B (<i>APOB</i>) gene was identified in the subsequent analysis. Experiment results and correlation analysis showed that the expression of <i>APOB</i> was higher in IM tissues than in normal tissues. This elevated expression of <i>APOB</i> was also found to be associated with the expression levels of hepatocyte nuclear factor 4A (<i>HNF4A</i>) gene. <i>HNF4A</i> was also found to be associated with immune cell infiltration to gastric cancer and was linked to the prognosis of gastric cancer patients. Moreover, <i>HNF4A</i> was also highly expressed in both IM tissues and gastric cancer cells.<h4>Conclusion</h4>Our findings indicate that <i>HNF4A</i> regulates the microenvironment of lipid metabolism in IM tissues by targeting <i>APOB</i>. Higher expression of <i>HNF4A</i> tends to lead to a worse prognosis in gastric cancer patients implying it may serve as a predictive indicator for the progression from IM to gastric cancer.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-07-01T03:23:57.491Z","creation":"2026-07-01T03:12:30.556Z"},"accession":"S-EPMC11047246","cross_references":{"pubmed":["38680615"],"doi":["10.1177/11769343241249017"]}}