biostudies-literatureDiaz MAAgencia Nacional de Promoción Científica y Técnica ANPCyTSCAIT-UNTNational University of TucumánConsejo Nacional de Investigaciones Científicas y TécnicasAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la InnovaciónConsejo Nacional de Investigaciones Científicas y Técnicas, CONICET297https://www.ebi.ac.uk/biostudies/studies/S-EPMC11047364biostudies-literatureUnknown13(4)The escalating prevalence of antibiotic-resistant bacteria poses a grave threat to human health, necessitating the exploration of novel alternatives to conventional antibiotics. This study investigated the impact of extracts derived from the supernatant of four lactic acid bacteria strains on factors contributing to the pathogenicity of three <i>Staphylococcus aureus</i> strains. The study evaluated the influence of lactic acid bacteria supernatant extracts on the growth, biofilm biomass formation, biofilm metabolic activity, and biofilm integrity of the <i>S. aureus</i> strains. Additionally, the impact on virulence factors (hemolysin and coagulase) was examined. Gas chromatography coupled with mass spectrometry was used to identify the bioactive compounds in the extracts, while molecularAntibiotics (Basel, Switzerland)Restraining <i>Staphylococcus aureus</i> Virulence Factors and <i>Quorum Sensing</i> through Lactic Acid Bacteria Supernatant Extracts.PMC11047364PICT 2018-02514PICT 2018-02071 and 02514, PICT 2021-0060, PICT-2021-I-A-00439 IPICT 2018-02071D708PICT-2021-I-A-00439 IPUE 0021PICT 2021-0060D702PIUNT D702, D708, and D715Vega-Hissi EGDiaz MABlazquez MAArena MEAlberto MRfalseRestraining <i>Staphylococcus aureus</i> Virulence Factors and <i>Quorum Sensing</i> through Lactic Acid Bacteria Supernatant Extracts.The escalating prevalence of antibiotic-resistant bacteria poses a grave threat to human health, necessitating the exploration of novel alternatives to conventional antibiotics. This study investigated the impact of extracts derived from the supernatant of four lactic acid bacteria strains on factors contributing to the pathogenicity of three <i>Staphylococcus aureus</i> strains. The study evaluated the influence of lactic acid bacteria supernatant extracts on the growth, biofilm biomass formation, biofilm metabolic activity, and biofilm integrity of the <i>S. aureus</i> strains. Additionally, the impact on virulence factors (hemolysin and coagulase) was examined. Gas chromatography coupled with mass spectrometry was used to identify the bioactive compounds in the extracts, while molecular2024-01-01T00:00:00Z2024 Mar2026-04-13T03:18:17.728Z2026-04-13T03:11:56.547ZS-EPMC110473643866697310.3390/antibiotics13040297