{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tan LL"],"funding":["Agency for Science, Technology and Research","Agency for Science, Technology and Research (A*STAR), Singapore","National Research Foundation","Singapore National Research Foundation (NRF)’s 19th Competitive Research Program"],"pagination":["486"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11048056"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(4)"],"pubmed_abstract":["In recent years, CRISPR-Cas toolboxes for <i>Streptomyces</i> editing have rapidly accelerated natural product discovery and engineering. However, Cas efficiencies are oftentimes strain-dependent, and the commonly used <i>Streptococcus pyogenes</i> Cas9 (SpCas9) is notorious for having high levels of off-target toxicity effects. Thus, a variety of Cas proteins is required for greater flexibility of genetic manipulation within a wider range of <i>Streptomyces</i> strains. This study explored the first use of <i>Acidaminococcus</i> sp. Cas12j, a hypercompact Cas12 subfamily, for genome editing in <i>Streptomyces</i> and its potential in activating silent biosynthetic gene clusters (BGCs) to enhance natural product synthesis. While the editing efficiencies of Cas12j were not as high as previo"],"journal":["Biomolecules"],"pubmed_title":["Application of Cas12j for <i>Streptomyces</i> Editing."],"pmcid":["PMC11048056"],"funding_grant_id":["C211917003","NRF-CRP19-2017-05","C233017003"],"pubmed_authors":["Yang LK","Kanagasundaram Y","Leong CY","Koduru L","Seow DCS","Heng E","Lim YH","Wong FT","Ng V","Ng SB","Peh G","Tan LL"],"additional_accession":[]},"is_claimable":false,"name":"Application of Cas12j for <i>Streptomyces</i> Editing.","description":"In recent years, CRISPR-Cas toolboxes for <i>Streptomyces</i> editing have rapidly accelerated natural product discovery and engineering. However, Cas efficiencies are oftentimes strain-dependent, and the commonly used <i>Streptococcus pyogenes</i> Cas9 (SpCas9) is notorious for having high levels of off-target toxicity effects. Thus, a variety of Cas proteins is required for greater flexibility of genetic manipulation within a wider range of <i>Streptomyces</i> strains. This study explored the first use of <i>Acidaminococcus</i> sp. Cas12j, a hypercompact Cas12 subfamily, for genome editing in <i>Streptomyces</i> and its potential in activating silent biosynthetic gene clusters (BGCs) to enhance natural product synthesis. While the editing efficiencies of Cas12j were not as high as previo","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-04-08T19:48:03.508Z","creation":"2026-04-08T14:30:54.872Z"},"accession":"S-EPMC11048056","cross_references":{"pubmed":["38672502"],"doi":["10.3390/biom14040486"]}}