biostudies-literatureUnknown15Zhang J<h4>Background</h4>Tuberculosis (TB), caused by <i>Mycobacterium tuberculosis</i> (Mtb), is a persistent infectious disease threatening human health. The existing diagnostic methods still have significant shortcomings, including a low positivity rate in pathogen-based diagnoses and the inability of immunological diagnostics to detect active TB. Hence, it is urgent to develop new techniques to detect TB more accurate and earlier. This research aims to scrutinize and authenticate DNA methylation markers suitable for tuberculosis diagnosis. Concurrently, Providing a new approach for tuberculosis diagnosis.<h4>Methods</h4>Blood samples from patients with newly diagnosed tuberculosis and healthy controls (HC) were utilized in this study. Examining methylation microarray data from 40 whole bloodFrontiers in genetics1380828https://www.ebi.ac.uk/biostudies/studies/S-EPMC11048481biostudies-literatureExploring TSPAN4 promoter methylation as a diagnostic biomarker for tuberculosis.PMC11048481Liu HZhang JChen JZhang YChen LZhang MYang QMo WfalseExploring TSPAN4 promoter methylation as a diagnostic biomarker for tuberculosis.<h4>Background</h4>Tuberculosis (TB), caused by <i>Mycobacterium tuberculosis</i> (Mtb), is a persistent infectious disease threatening human health. The existing diagnostic methods still have significant shortcomings, including a low positivity rate in pathogen-based diagnoses and the inability of immunological diagnostics to detect active TB. Hence, it is urgent to develop new techniques to detect TB more accurate and earlier. This research aims to scrutinize and authenticate DNA methylation markers suitable for tuberculosis diagnosis. Concurrently, Providing a new approach for tuberculosis diagnosis.<h4>Methods</h4>Blood samples from patients with newly diagnosed tuberculosis and healthy controls (HC) were utilized in this study. Examining methylation microarray data from 40 whole blood2024-01-01T00:00:00Z20242026-04-24T03:14:58.895Z2026-04-24T03:09:37.801ZS-EPMC110484813868042110.3389/fgene.2024.1380828