{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Che L"],"funding":["Key Project of the Natural Science Foundation of Gansu Province","FuXi Foundation of Gansu Agricultural University","FuXi Foundation of the Gansu Agricultural University","Key Project of Natural Science Foundation of Gansu Province"],"pagination":["4458"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11049880"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(8)"],"pubmed_abstract":["Cold stress adversely impacts grape growth, development, and yield. Therefore, improving the cold tolerance of grape is an urgent task of grape breeding. The Jasmonic acid (JA) pathway responsive gene <i>JAZ</i> plays a key role in plant response to cold stress. However, the role of <i>JAZ</i> in response to low temperatures in grape is unclear. In this study, <i>VvJAZ13</i> was cloned from the 'Pinot Noir' (<i>Vitis vinefera</i> cv. 'Pinot Noir') grape, and the potential interacting protein of VvJAZ13 was screened by yeast two-hybrid (Y2H). The function of <i>VvJAZ13</i> under low temperature stress was verified by genetic transformation. Subcellular localization showed that the gene was mainly expressed in cytoplasm and the nucleus. Y2H indicated that VvF-box, VvTIFY5A, VvTIFY9, Vvbch1, and VvAGD13 may be potential interacting proteins of VvJAZ13. The results of transient transformation of grape leaves showed that <i>VvJAZ13</i> improved photosynthetic capacity and reduced cell damage by increasing maximum photosynthetic efficiency of photosystem II (Fv/Fm), reducing relative electrolyte leakage (REL) and malondialdehyde (MDA), and increasing proline content in overexpressed lines (OEs), which played an active role in cold resistance. Through the overexpression of <i>VvJAZ13</i> in <i>Arabidopsis thaliana</i> and grape calli, the results showed that compared with wild type (WT), transgenic lines had higher antioxidant enzyme activity and proline content, lower REL, MDA, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, and an improved ability of scavenging reactive oxygen species. In addition, the expression levels of <i>CBF1-2</i> and <i>ICE1</i> genes related to cold response were up-regulated in transgenic lines. To sum up, <i>VvJAZ13</i> is actively involved in the cold tolerance of <i>Arabidopsis</i> and grape, and has the potential to be a candidate gene for improving plant cold tolerance."],"journal":["International journal of molecular sciences"],"pubmed_title":["<i>VvJAZ13</i> Positively Regulates Cold Tolerance in <i>Arabidopsis</i> and Grape."],"pmcid":["PMC11049880"],"funding_grant_id":["Ganfx-03J02","22JR5RA831","No. Ganfx-03J02"],"pubmed_authors":["Guo L","Yang J","Lu S","Gou H","Che L","Li M","Mao J"],"additional_accession":[]},"is_claimable":false,"name":"<i>VvJAZ13</i> Positively Regulates Cold Tolerance in <i>Arabidopsis</i> and Grape.","description":"Cold stress adversely impacts grape growth, development, and yield. Therefore, improving the cold tolerance of grape is an urgent task of grape breeding. The Jasmonic acid (JA) pathway responsive gene <i>JAZ</i> plays a key role in plant response to cold stress. However, the role of <i>JAZ</i> in response to low temperatures in grape is unclear. In this study, <i>VvJAZ13</i> was cloned from the 'Pinot Noir' (<i>Vitis vinefera</i> cv. 'Pinot Noir') grape, and the potential interacting protein of VvJAZ13 was screened by yeast two-hybrid (Y2H). The function of <i>VvJAZ13</i> under low temperature stress was verified by genetic transformation. Subcellular localization showed that the gene was mainly expressed in cytoplasm and the nucleus. Y2H indicated that VvF-box, VvTIFY5A, VvTIFY9, Vvbch1, and VvAGD13 may be potential interacting proteins of VvJAZ13. The results of transient transformation of grape leaves showed that <i>VvJAZ13</i> improved photosynthetic capacity and reduced cell damage by increasing maximum photosynthetic efficiency of photosystem II (Fv/Fm), reducing relative electrolyte leakage (REL) and malondialdehyde (MDA), and increasing proline content in overexpressed lines (OEs), which played an active role in cold resistance. Through the overexpression of <i>VvJAZ13</i> in <i>Arabidopsis thaliana</i> and grape calli, the results showed that compared with wild type (WT), transgenic lines had higher antioxidant enzyme activity and proline content, lower REL, MDA, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, and an improved ability of scavenging reactive oxygen species. In addition, the expression levels of <i>CBF1-2</i> and <i>ICE1</i> genes related to cold response were up-regulated in transgenic lines. To sum up, <i>VvJAZ13</i> is actively involved in the cold tolerance of <i>Arabidopsis</i> and grape, and has the potential to be a candidate gene for improving plant cold tolerance.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-29T08:11:46.881Z","creation":"2026-06-29T03:09:24.967Z"},"accession":"S-EPMC11049880","cross_references":{"pubmed":["38674041"],"doi":["10.3390/ijms25084458"]}}