biostudies-literatureThapa BBNational Research Foundation (NRF) of KoreaNational Research Foundation of KoreaUniversity Grants Commission, Nepal4193https://www.ebi.ac.uk/biostudies/studies/S-EPMC11050201biostudies-literatureUnknown25(8)<i>Streptomyces</i> are well-known for producing bioactive secondary metabolites, with numerous antimicrobials essential to fight against infectious diseases. Globally, multidrug-resistant (MDR) microorganisms significantly challenge human and veterinary diseases. To tackle this issue, there is an urgent need for alternative antimicrobials. In the search for potent agents, we have isolated four <i>Streptomyces</i> species PC1, BT1, BT2, and BT3 from soils collected from various geographical regions of the Himalayan country Nepal, which were then identified based on morphology and 16S rRNA gene sequencing. The relationship of soil microbes with different <i>Streptomyces</i> species has been shown in phylogenetic trees. Antimicrobial potency of isolates was carried out against <i>StaphylococInternational journal of molecular sciencesMetabolic Comparison and Molecular Networking of Antimicrobials in <i>Streptomyces</i> Species.PMC110502012019R1A5A2027340CRIG-78/79-S&amp;T-012021R1A2C20079372019R1A5A2027340 and 2021R1A2C2007937RS-2023-00259697Chaudhary PThapa BBSohng JKHuo CBudhathoki RMagar RTKim KHJoshi SPoudel PBParajuli NfalseMetabolic Comparison and Molecular Networking of Antimicrobials in <i>Streptomyces</i> Species.<i>Streptomyces</i> are well-known for producing bioactive secondary metabolites, with numerous antimicrobials essential to fight against infectious diseases. Globally, multidrug-resistant (MDR) microorganisms significantly challenge human and veterinary diseases. To tackle this issue, there is an urgent need for alternative antimicrobials. In the search for potent agents, we have isolated four <i>Streptomyces</i> species PC1, BT1, BT2, and BT3 from soils collected from various geographical regions of the Himalayan country Nepal, which were then identified based on morphology and 16S rRNA gene sequencing. The relationship of soil microbes with different <i>Streptomyces</i> species has been shown in phylogenetic trees. Antimicrobial potency of isolates was carried out against <i>Staphylococ2024-01-01T00:00:00Z2024 Apr2026-05-20T03:16:03.512Z2026-05-20T03:07:44.583ZS-EPMC110502013867377710.3390/ijms25084193