biostudies-literatureLi KNational Key R&D Program of ChinaQingdao New Energy Shandong LaboratoryNatural Science Foundation of Shandong ProvinceYoung Taishan ScholarsYouth Innovation Promotion Association CAS663https://www.ebi.ac.uk/biostudies/studies/S-EPMC11052032biostudies-literatureUnknown12(4)2-Phenylethanol (2-PE) and indole-3-acetic acid (IAA) are important secondary metabolites produced by microorganisms, and their production are closely linked to the growth state of microorganisms and environmental factors. <i>Enterobacter</i> CGMCC 5087 can produce both 2-PE and IAA depending on α-ketoacid decarboxylase KDC4427. This study aimed to investigate the effects of different environment factors including osmotic pressure, temperature, and pH on the synthesis of 2-PE and IAA in <i>Enterobacter</i> sp. CGMCC 5087. The bacteria exhibited an enhanced capacity for 2-PE synthesis while not affecting IAA synthesis under 5% NaCl and pH 4.5 stress conditions. In an environment with pH 9.5, the synthesis capacity of 2-PE remained unchanged while the synthesis capacity of IAA decreased. TheMicroorganismsEffects of Environmental Stresses on Synthesis of 2-Phenylethanol and IAA by <i>Enterobacter</i> sp. CGMCC 5087.PMC11052032QNESL 0P202308TSQN201909159Y20210632022YFC2106200ZR2023MC163Liu LZhang XWu PZhang HZheng RFang SWei XLi KfalseEffects of Environmental Stresses on Synthesis of 2-Phenylethanol and IAA by <i>Enterobacter</i> sp. CGMCC 5087.2-Phenylethanol (2-PE) and indole-3-acetic acid (IAA) are important secondary metabolites produced by microorganisms, and their production are closely linked to the growth state of microorganisms and environmental factors. <i>Enterobacter</i> CGMCC 5087 can produce both 2-PE and IAA depending on α-ketoacid decarboxylase KDC4427. This study aimed to investigate the effects of different environment factors including osmotic pressure, temperature, and pH on the synthesis of 2-PE and IAA in <i>Enterobacter</i> sp. CGMCC 5087. The bacteria exhibited an enhanced capacity for 2-PE synthesis while not affecting IAA synthesis under 5% NaCl and pH 4.5 stress conditions. In an environment with pH 9.5, the synthesis capacity of 2-PE remained unchanged while the synthesis capacity of IAA decreased. The2024-01-01T00:00:00Z2024 Mar2026-04-08T15:21:38.484Z2026-04-08T04:27:26.61ZS-EPMC110520323867460710.3390/microorganisms12040663