biostudies-literatureUnknown15Deng AAt present, it is widely believed that a 95-96% average nucleotide identity (ANI) value is equivalent to a 70% digital DNA-DNA hybridization (dDDH) value in the prokaryotic taxonomy. However, in the present study, comparative genome analysis of 29 pairs of <i>Amycolatopsis</i> type strains revealed that a 70% dDDH value did not correspond to a 95-96% ANI based on the MuMmer ultra-rapid aligning tool (ANIm) but approximately corresponded to a 96.6% ANIm value in the genus <i>Amycolatopsis</i>. Based on this corresponding relationship, phenotypic and chemotaxonomical characteristics, as well as phylogenetic analysis, an actinobacterial strain HUAS 11-8^T isolated from the rhizosphere soil of <i>Cynara scolymus</i>, was subjected to a polyphasic taxonomic characterization. Based on Frontiers in microbiology1359021https://www.ebi.ac.uk/biostudies/studies/S-EPMC11056570biostudies-literatureNew insights into the relationship between the average nucleotide identity and the digital DNA-DNA hybridization values in the genus <i>Amycolatopsis</i> and <i>Amycolatopsis cynarae</i> sp. nov., a novel actinobacterium from the rhizosphere soil of <i>Cynara scolymus</i>, and proposal of <i>Amycolatopsis niigatensis</i> as a synonym of <i>Amycolatopsis echigonensis</i> based on comparative genomic analysis.PMC11056570Zheng YMo PDeng AGao JFu LTang TfalseNew insights into the relationship between the average nucleotide identity and the digital DNA-DNA hybridization values in the genus <i>Amycolatopsis</i> and <i>Amycolatopsis cynarae</i> sp. nov., a novel actinobacterium from the rhizosphere soil of <i>Cynara scolymus</i>, and proposal of <i>Amycolatopsis niigatensis</i> as a synonym of <i>Amycolatopsis echigonensis</i> based on comparative genomic analysis.At present, it is widely believed that a 95-96% average nucleotide identity (ANI) value is equivalent to a 70% digital DNA-DNA hybridization (dDDH) value in the prokaryotic taxonomy. However, in the present study, comparative genome analysis of 29 pairs of <i>Amycolatopsis</i> type strains revealed that a 70% dDDH value did not correspond to a 95-96% ANI based on the MuMmer ultra-rapid aligning tool (ANIm) but approximately corresponded to a 96.6% ANIm value in the genus <i>Amycolatopsis</i>. Based on this corresponding relationship, phenotypic and chemotaxonomical characteristics, as well as phylogenetic analysis, an actinobacterial strain HUAS 11-8^T isolated from the rhizosphere soil of <i>Cynara scolymus</i>, was subjected to a polyphasic taxonomic characterization. Based on 2024-01-01T00:00:00Z20242026-04-30T21:22:01.732Z2026-04-07T16:21:28.507ZS-EPMC110565703868611010.3389/fmicb.2024.1359021