{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23"],"submitter":["Yi L"],"funding":["National Natural Science Foundation of China"],"pubmed_abstract":["Camelids produce both conventional tetrameric antibodies (Abs) and dimeric heavy-chain antibodies (HCAbs). Although B cells that generate these two types of Abs exhibit distinct B cell receptors (BCRs), whether these two B cell populations differ in their phenotypes and developmental processes remains unclear. Here, we performed single-cell 5' RNA profiling of peripheral blood mononuclear cell samples from Bactrian camels before and after immunization. We characterized the functional subtypes and differentiation trajectories of circulating B cells in camels, and reconstructed single-cell BCR sequences. We found that in contrast to humans, the proportion of T-bet+ B cells was high among camelid peripheral B cells. Several marker genes of human B cell subtypes, including <i>CD27</i> and <i>IGHD</i>, were expressed at low levels in the corresponding camel B cell subtypes. Camelid B cells expressing variable genes of HACbs (<i>VHH</i>) were widely present in various functional subtypes and showed highly overlapping differentiation trajectories with B cells expressing variable genes of conventional Abs (<i>VH</i>). After immunization, the transcriptional changes in <i>VHH</i>+ and <i>VH</i>+ B cells were largely consistent. Through structure modeling, we identified a variety of scaffold types among the reconstructed VHH sequences. Our study provides insights into the cellular context of HCAb production in camels and lays the foundation for developing single-B cell-based camelid single-domain Ab screening."],"journal":["Computational and structural biotechnology journal"],"pagination":["1705-1714"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11059136"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell 5' RNA sequencing of camelid peripheral B cells provides insights into cellular basis of heavy-chain antibody production."],"pmcid":["PMC11059136"],"pubmed_authors":["Liu Y","Yi L","Guo X","Jirimutu","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell 5' RNA sequencing of camelid peripheral B cells provides insights into cellular basis of heavy-chain antibody production.","description":"Camelids produce both conventional tetrameric antibodies (Abs) and dimeric heavy-chain antibodies (HCAbs). Although B cells that generate these two types of Abs exhibit distinct B cell receptors (BCRs), whether these two B cell populations differ in their phenotypes and developmental processes remains unclear. Here, we performed single-cell 5' RNA profiling of peripheral blood mononuclear cell samples from Bactrian camels before and after immunization. We characterized the functional subtypes and differentiation trajectories of circulating B cells in camels, and reconstructed single-cell BCR sequences. We found that in contrast to humans, the proportion of T-bet+ B cells was high among camelid peripheral B cells. Several marker genes of human B cell subtypes, including <i>CD27</i> and <i>IGHD</i>, were expressed at low levels in the corresponding camel B cell subtypes. Camelid B cells expressing variable genes of HACbs (<i>VHH</i>) were widely present in various functional subtypes and showed highly overlapping differentiation trajectories with B cells expressing variable genes of conventional Abs (<i>VH</i>). After immunization, the transcriptional changes in <i>VHH</i>+ and <i>VH</i>+ B cells were largely consistent. Through structure modeling, we identified a variety of scaffold types among the reconstructed VHH sequences. Our study provides insights into the cellular context of HCAb production in camels and lays the foundation for developing single-B cell-based camelid single-domain Ab screening.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-05-29T12:07:12.849Z","creation":"2026-04-08T04:41:40.977Z"},"accession":"S-EPMC11059136","cross_references":{"pubmed":["38689719"],"doi":["10.1016/j.csbj.2024.04.041"]}}