biostudies-literatureGonzalez-de-Miguel JEuropean Research Council124https://www.ebi.ac.uk/biostudies/studies/S-EPMC11059754biostudies-literatureUnknown23(1)<h4>Background</h4>Having a simple and fast dividing organism capable of producing and exposing at its surface or secreting functional complex biomolecules with disulphide bridges is of great interest. The mycoplasma bacterial genus offers a set of relevant properties that make it an interesting chassis for such purposes, the main one being the absence of a cell wall. However, due to their slow growth, they have rarely been considered as a potential platform in this respect. This notion may be challenged with the recent discovery of Mycoplasma feriruminatoris, a species with a dividing time close to that of common microbial workhorses. So far, no tools for heterologous protein expression nor secretion have been described for it.<h4>Results</h4>The work presented here develops the fast-diviMicrobial cell factoriesDeveloping a platform for secretion of biomolecules in Mycoplasma feriruminatoris.PMC11059754101020135Ciampi LGonzalez-de-Miguel JRodriguez-Arce ISerrano LMontero-Blay AfalseDeveloping a platform for secretion of biomolecules in Mycoplasma feriruminatoris.<h4>Background</h4>Having a simple and fast dividing organism capable of producing and exposing at its surface or secreting functional complex biomolecules with disulphide bridges is of great interest. The mycoplasma bacterial genus offers a set of relevant properties that make it an interesting chassis for such purposes, the main one being the absence of a cell wall. However, due to their slow growth, they have rarely been considered as a potential platform in this respect. This notion may be challenged with the recent discovery of Mycoplasma feriruminatoris, a species with a dividing time close to that of common microbial workhorses. So far, no tools for heterologous protein expression nor secretion have been described for it.<h4>Results</h4>The work presented here develops the fast-divi2024-01-01T00:00:00Z2024 Apr2026-06-02T22:47:34.97Z2025-08-30T03:06:04.181ZS-EPMC110597543868925110.1186/s12934-024-02392-3