{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Midha MK"],"funding":["NIA NIH HHS","NIH Office of the Director","Division of Biological Infrastructure","National Heart, Lung, and Blood Institute","NHLBI NIH HHS","NIH HHS","National Institute on Aging"],"pagination":["2525-2537"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11060334"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(7)"],"pubmed_abstract":["By far the largest contribution to ion detectability in liquid chromatography-driven mass spectrometry-based proteomics is the efficient generation of peptide molecular ions by the electrospray source. To maximize the transfer of peptides from the liquid to gaseous phase and allow molecular ions to enter the mass spectrometer at microspray flow rates, an efficient electrospray process is required. Here we describe the superior performance of newly design vacuum insulated probe heated electrospray ionization (VIP-HESI) source coupled to a Bruker timsTOF PRO mass spectrometer operated in microspray mode. VIP-HESI significantly improves chromatography signals in comparison to electrospray ionization (ESI) and nanospray ionization using the captivespray (CS) source and provides increased prote"],"journal":["Journal of proteome research"],"pubmed_title":["Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer."],"pmcid":["PMC11060334"],"funding_grant_id":["U19AG023122","S10 OD026936","1920268","R01 HL133135","R01HL133135","U19 AG023122","S10OD026936"],"pubmed_authors":["Maes M","Baxter DH","Kapil C","Prokop TJ","Midha MK","Morrone SR","Moritz RL"],"additional_accession":[]},"is_claimable":false,"name":"Vacuum Insulated Probe Heated Electrospray Ionization Source Enhances Microflow Rate Chromatography Signals in the Bruker timsTOF Mass Spectrometer.","description":"By far the largest contribution to ion detectability in liquid chromatography-driven mass spectrometry-based proteomics is the efficient generation of peptide molecular ions by the electrospray source. To maximize the transfer of peptides from the liquid to gaseous phase and allow molecular ions to enter the mass spectrometer at microspray flow rates, an efficient electrospray process is required. Here we describe the superior performance of newly design vacuum insulated probe heated electrospray ionization (VIP-HESI) source coupled to a Bruker timsTOF PRO mass spectrometer operated in microspray mode. VIP-HESI significantly improves chromatography signals in comparison to electrospray ionization (ESI) and nanospray ionization using the captivespray (CS) source and provides increased prote","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2026-05-29T11:45:08.66Z","creation":"2025-04-04T12:21:17.609Z"},"accession":"S-EPMC11060334","cross_references":{"pubmed":["37294184"],"doi":["10.1021/acs.jproteome.3c00305"]}}