{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(2)"],"submitter":["Van den Langenbergh J"],"funding":["Gigtforeningen"],"pubmed_abstract":["<h4>Objective</h4>To investigate associations between obesity-linked systemic factors and gene expression indicative for the inflammatory and fibrotic processes in the infrapatellar fat pad (IFP), in a population of obese patients with end-stage knee osteoarthritis (KOA).<h4>Methods</h4>We collected human IFPs from 48 patients with a mean body mass index (BMI) of 35.44 ​kg/m<sup>2</sup> during total knee replacement procedures. These patients were part of a randomized controlled trial and met the criteria of having OA and a BMI of ≥30 ​kg/m<sup>2</sup>. Blood samples were collected to assess serum levels of glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and leptin. Total body composition was measured using dual-energy X-ray absorptiometry. Gene expressions of <i>IL6, TNFA, COL1A1, IL1B, ASMA, PLOD2</i> in the IFP were analyzed.<h4>Results</h4>Univariate analysis resulted in a positive correlation between BMI and procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (<i>PLOD2</i>) expression (<i>r</i><sup>2</sup> ​= ​0.13). In univariate analyses of obesity-linked systemic factors and <i>PLOD2</i>, significant correlations were found for lean mass (<i>r</i><sup>2</sup> ​= ​0.20), fat mass (<i>r</i><sup>2</sup> ​= ​0.20), serum cholesterol (<i>r</i><sup>2</sup> ​= ​0.17), serum triglycerides (<i>r</i><sup>2</sup> ​= ​0.19) and serum leptin (<i>r</i><sup>2</sup> ​= ​0.10). A multiple linear regression model indicated fat mass to be a strong predictor of PLOD2 production in the IFP (<i>r</i><sup>2</sup> ​= ​0.22, <i>P</i> ​= ​0.003).<h4>Conclusion</h4>Our study demonstrates the positive association between fat mass and <i>PLOD2</i> expression in the IFP of obese end-stage knee OA patients. This may indicate that within this patient population the fibrotic process in the IFP is influenced by systemic adipose tissue, next to local inflammatory processes."],"journal":["Osteoarthritis and cartilage open"],"pagination":["100469"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11061337"],"repository":["biostudies-literature"],"pubmed_title":["<i>PLOD2</i> gene expression in infrapatellar fat pad is correlated with fat mass in obese patients with end-stage knee osteoarthritis."],"pmcid":["PMC11061337"],"pubmed_authors":["Runhaar J","Mechlenburg I","Liljensoe A","Bierma-Zeinstra SMA","van Osch GJVM","Van den Langenbergh J","Kops N","Soballe K","Bastiaansen-Jenniskens YM","Clockaerts S","Laursen J"],"additional_accession":[]},"is_claimable":false,"name":"<i>PLOD2</i> gene expression in infrapatellar fat pad is correlated with fat mass in obese patients with end-stage knee osteoarthritis.","description":"<h4>Objective</h4>To investigate associations between obesity-linked systemic factors and gene expression indicative for the inflammatory and fibrotic processes in the infrapatellar fat pad (IFP), in a population of obese patients with end-stage knee osteoarthritis (KOA).<h4>Methods</h4>We collected human IFPs from 48 patients with a mean body mass index (BMI) of 35.44 ​kg/m<sup>2</sup> during total knee replacement procedures. These patients were part of a randomized controlled trial and met the criteria of having OA and a BMI of ≥30 ​kg/m<sup>2</sup>. Blood samples were collected to assess serum levels of glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and leptin. Total body composition was measured using dual-energy X-ray absorptiometry. Gene expressions of <i>IL6, TNFA, COL1A1, IL1B, ASMA, PLOD2</i> in the IFP were analyzed.<h4>Results</h4>Univariate analysis resulted in a positive correlation between BMI and procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (<i>PLOD2</i>) expression (<i>r</i><sup>2</sup> ​= ​0.13). In univariate analyses of obesity-linked systemic factors and <i>PLOD2</i>, significant correlations were found for lean mass (<i>r</i><sup>2</sup> ​= ​0.20), fat mass (<i>r</i><sup>2</sup> ​= ​0.20), serum cholesterol (<i>r</i><sup>2</sup> ​= ​0.17), serum triglycerides (<i>r</i><sup>2</sup> ​= ​0.19) and serum leptin (<i>r</i><sup>2</sup> ​= ​0.10). A multiple linear regression model indicated fat mass to be a strong predictor of PLOD2 production in the IFP (<i>r</i><sup>2</sup> ​= ​0.22, <i>P</i> ​= ​0.003).<h4>Conclusion</h4>Our study demonstrates the positive association between fat mass and <i>PLOD2</i> expression in the IFP of obese end-stage knee OA patients. This may indicate that within this patient population the fibrotic process in the IFP is influenced by systemic adipose tissue, next to local inflammatory processes.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-29T12:07:11.401Z","creation":"2026-04-08T04:41:37.944Z"},"accession":"S-EPMC11061337","cross_references":{"pubmed":["38694906"],"doi":["10.1016/j.ocarto.2024.100469"]}}