biostudies-literatureUnknown6(2)Van den Langenbergh JGigtforeningen<h4>Objective</h4>To investigate associations between obesity-linked systemic factors and gene expression indicative for the inflammatory and fibrotic processes in the infrapatellar fat pad (IFP), in a population of obese patients with end-stage knee osteoarthritis (KOA).<h4>Methods</h4>We collected human IFPs from 48 patients with a mean body mass index (BMI) of 35.44 ​kg/m² during total knee replacement procedures. These patients were part of a randomized controlled trial and met the criteria of having OA and a BMI of ≥30 ​kg/m². Blood samples were collected to assess serum levels of glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and leptin. Total body composition was measured using dual-energy X-ray absorptiometry. Gene expressions of <i>IL6, TNFA, COL1A1, IL1B, ASMA, PLOD2</i> in the IFP were analyzed.<h4>Results</h4>Univariate analysis resulted in a positive correlation between BMI and procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (<i>PLOD2</i>) expression (<i>r</i>² ​= ​0.13). In univariate analyses of obesity-linked systemic factors and <i>PLOD2</i>, significant correlations were found for lean mass (<i>r</i>² ​= ​0.20), fat mass (<i>r</i>² ​= ​0.20), serum cholesterol (<i>r</i>² ​= ​0.17), serum triglycerides (<i>r</i>² ​= ​0.19) and serum leptin (<i>r</i>² ​= ​0.10). A multiple linear regression model indicated fat mass to be a strong predictor of PLOD2 production in the IFP (<i>r</i>² ​= ​0.22, <i>P</i> ​= ​0.003).<h4>Conclusion</h4>Our study demonstrates the positive association between fat mass and <i>PLOD2</i> expression in the IFP of obese end-stage knee OA patients. This may indicate that within this patient population the fibrotic process in the IFP is influenced by systemic adipose tissue, next to local inflammatory processes.Osteoarthritis and cartilage open100469https://www.ebi.ac.uk/biostudies/studies/S-EPMC11061337biostudies-literature<i>PLOD2</i> gene expression in infrapatellar fat pad is correlated with fat mass in obese patients with end-stage knee osteoarthritis.PMC11061337Runhaar JMechlenburg ILiljensoe ABierma-Zeinstra SMAvan Osch GJVMVan den Langenbergh JKops NSoballe KBastiaansen-Jenniskens YMClockaerts SLaursen Jfalse<i>PLOD2</i> gene expression in infrapatellar fat pad is correlated with fat mass in obese patients with end-stage knee osteoarthritis.<h4>Objective</h4>To investigate associations between obesity-linked systemic factors and gene expression indicative for the inflammatory and fibrotic processes in the infrapatellar fat pad (IFP), in a population of obese patients with end-stage knee osteoarthritis (KOA).<h4>Methods</h4>We collected human IFPs from 48 patients with a mean body mass index (BMI) of 35.44 ​kg/m² during total knee replacement procedures. These patients were part of a randomized controlled trial and met the criteria of having OA and a BMI of ≥30 ​kg/m². Blood samples were collected to assess serum levels of glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, and leptin. Total body composition was measured using dual-energy X-ray absorptiometry. Gene expressions of <i>IL6, TNFA, COL1A1, IL1B, ASMA, PLOD2</i> in the IFP were analyzed.<h4>Results</h4>Univariate analysis resulted in a positive correlation between BMI and procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 (<i>PLOD2</i>) expression (<i>r</i>² ​= ​0.13). In univariate analyses of obesity-linked systemic factors and <i>PLOD2</i>, significant correlations were found for lean mass (<i>r</i>² ​= ​0.20), fat mass (<i>r</i>² ​= ​0.20), serum cholesterol (<i>r</i>² ​= ​0.17), serum triglycerides (<i>r</i>² ​= ​0.19) and serum leptin (<i>r</i>² ​= ​0.10). A multiple linear regression model indicated fat mass to be a strong predictor of PLOD2 production in the IFP (<i>r</i>² ​= ​0.22, <i>P</i> ​= ​0.003).<h4>Conclusion</h4>Our study demonstrates the positive association between fat mass and <i>PLOD2</i> expression in the IFP of obese end-stage knee OA patients. This may indicate that within this patient population the fibrotic process in the IFP is influenced by systemic adipose tissue, next to local inflammatory processes.2024-01-01T00:00:00Z2024 Jun2026-05-29T12:07:11.401Z2026-04-08T04:41:37.944ZS-EPMC110613373869490610.1016/j.ocarto.2024.100469