biostudies-literatureUnknown86(4)Fujino KChicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 in debilitated chickens with hemorrhagic hepatitis at a broiler farm in Japan. After euthanasia and necropsy, liver hemorrhage was observed. Nuclear inclusion bodies in the hepatocytes were identified using histological analysis. High-throughput sequencing analysis using RNA from livers of three affected chickens revealed infection by chicken chapparvovirus 2 and chicken anemia virus. Polymerase chain reaction analysis showed that all three chickens were positive for chicken chapparvovirus 2, and only one was positive for both chicken chapparvovirus 2 and chicken anemia virus. In conclusion, chicken chapparvovirus 2 causes infection in chicThe Journal of veterinary medical science396-399https://www.ebi.ac.uk/biostudies/studies/S-EPMC11061579biostudies-literatureDetection of chicken chapparvovirus 2 in chickens with hemorrhagic hepatitis in Japan.PMC11061579Aihara NFujino KKamiie JHorie MTaharaguchi SfalseDetection of chicken chapparvovirus 2 in chickens with hemorrhagic hepatitis in Japan.Chicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 in debilitated chickens with hemorrhagic hepatitis at a broiler farm in Japan. After euthanasia and necropsy, liver hemorrhage was observed. Nuclear inclusion bodies in the hepatocytes were identified using histological analysis. High-throughput sequencing analysis using RNA from livers of three affected chickens revealed infection by chicken chapparvovirus 2 and chicken anemia virus. Polymerase chain reaction analysis showed that all three chickens were positive for chicken chapparvovirus 2, and only one was positive for both chicken chapparvovirus 2 and chicken anemia virus. In conclusion, chicken chapparvovirus 2 causes infection in chic2024-01-01T00:00:00Z2024 Apr2026-05-29T11:23:32.753Z2026-04-08T04:29:41.675ZS-EPMC110615793834672610.1292/jvms.23-0415