biostudies-literatureRen YHainan Natural Science FoundationEducation Department of Hainan Province563-567https://www.ebi.ac.uk/biostudies/studies/S-EPMC11062271biostudies-literatureUnknown9(5)<i>Graptemys ouachitensis</i> (CAGLE, 1953) belongs to the <i>Graptemys</i> genus, <i>the</i> Emydidae family, and the Testudines order. This study involved sequencing the complete mitochondrial genome (mitogenome) of <i>G. ouachitensis</i> using next-generation sequencing, and analyzing the essential characteristics, and phylogenetic relationship. The results revealed that the <i>G. ouachitensis</i> mitogenome was 16,674 bp in length (A: 34.1%, C: 26.0%, G: 13.0%, T: 26.9%) and included 22 <i>tRNAs</i>, 13 protein-coding genes, two ribosomal RNA genes, and a non-coding control region (GenBank accession: NC071766). The genome composition of <i>G. ouachitensis</i> presented a slight A + T bias (61.0%) and exhibited a positive AT skew (0.118) and a negative GC skew (-0.333). A phylogenetic aMitochondrial DNA. Part B, ResourcesThe characterization of the mitochondrial genome of <i>Graptemys ouachitensis</i>.PMC11062271320QN256Hnky2021-25Zuo ZZhai XRen YXing XWang TMa GfalseThe characterization of the mitochondrial genome of <i>Graptemys ouachitensis</i>.<i>Graptemys ouachitensis</i> (CAGLE, 1953) belongs to the <i>Graptemys</i> genus, <i>the</i> Emydidae family, and the Testudines order. This study involved sequencing the complete mitochondrial genome (mitogenome) of <i>G. ouachitensis</i> using next-generation sequencing, and analyzing the essential characteristics, and phylogenetic relationship. The results revealed that the <i>G. ouachitensis</i> mitogenome was 16,674 bp in length (A: 34.1%, C: 26.0%, G: 13.0%, T: 26.9%) and included 22 <i>tRNAs</i>, 13 protein-coding genes, two ribosomal RNA genes, and a non-coding control region (GenBank accession: NC071766). The genome composition of <i>G. ouachitensis</i> presented a slight A + T bias (61.0%) and exhibited a positive AT skew (0.118) and a negative GC skew (-0.333). A phylogenetic a2024-01-01T00:00:00Z20242026-04-08T23:13:20.152Z2026-04-07T13:17:57.632ZS-EPMC110622713869952110.1080/23802359.2024.2347506