biostudies-literatureUnknown11(1)Jensen RODOE | SC | Biological and Environmental ResearchU.S. Department of Energy (DOE), Office of Science (SC), Office of Biological and Environmental ResearchThe genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes), whose members can encode useful complex traits such as solvent production, gas-fermentation, and lignocellulose breakdown. We describe 270 genome sequences of solventogenic clostridia from a comprehensive industrial strain collection assembled by Professor David Jones that includes 194 C. beijerinckii, 57 C. saccharobutylicum, 4 C. saccharoperbutylacetonicum, 5 C. butyricum, 7 C. acetobutylicum, and 3 C. tetanomorphum genomes. We report methods, analyses and characterization for phylogeny, key attributes, core biosynthetic genes, secondary metabolites, plasmids, prophage/CRISPR diversity, cellulosomes and quorum sensing for the 6 species. The expanded genomic data described here will facilitate eScientific data432https://www.ebi.ac.uk/biostudies/studies/S-EPMC11063209biostudies-literaturePhylogenomics and genetic analysis of solvent-producing Clostridium species.PMC11063209Reynoso VDe Tissera SBrown SDKopke MWoyke TReddy TBKMizrahi INagaraju SMorais SMouncey NJShapiro NRoux SUdwary DKlingeman DMMitchell WPWinkler JSchulz FJewett MCUtturkar SMJones DTIvanova NBayer EAJensen ROSimpson SDfalsePhylogenomics and genetic analysis of solvent-producing Clostridium species.The genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes), whose members can encode useful complex traits such as solvent production, gas-fermentation, and lignocellulose breakdown. We describe 270 genome sequences of solventogenic clostridia from a comprehensive industrial strain collection assembled by Professor David Jones that includes 194 C. beijerinckii, 57 C. saccharobutylicum, 4 C. saccharoperbutylacetonicum, 5 C. butyricum, 7 C. acetobutylicum, and 3 C. tetanomorphum genomes. We report methods, analyses and characterization for phylogeny, key attributes, core biosynthetic genes, secondary metabolites, plasmids, prophage/CRISPR diversity, cellulosomes and quorum sensing for the 6 species. The expanded genomic data described here will facilitate e2024-01-01T00:00:00Z2024 May2026-04-08T18:53:09.103Z2026-04-08T11:01:46.98ZS-EPMC110632093869319110.1038/s41597-024-03210-6