biostudies-literatureUnknown11Lamas-Paz AWellcome Trust<h4>Background</h4>Alcohol misuse, binge drinking pattern, and gender-specific effects in the middle-aged population has been clearly underestimated. In the present study, we focused on understanding gender-specific effects of alcohol exposure on the gut-liver axis and the role of gut microbiota in modulating gender-specific responses to alcohol consumption.<h4>Methods</h4>Fifty-two-week-old female and male C57BL/6 mice were fasted for 12 h, and then administered a single oral dose of ethanol (EtOH) (6 g/kg). Controls were given a single dose of PBS. Animals were sacrificed 8 h later. Alternatively, fecal microbiota transplantation (FMT) was performed in 52-week-old male mice from female donors of the same age. Permeability of the large intestine (colon), gut microbiota, liver injury, and Frontiers in nutrition1393014https://www.ebi.ac.uk/biostudies/studies/S-EPMC11063254biostudies-literatureFecal microbiota transplantation from female donors restores gut permeability and reduces liver injury and inflammation in middle-aged male mice exposed to alcohol.PMC11063254Gutierrez AHBanares RRoa SMesquita MVaquero JBenede-Ubieto REstevez-Vazquez OWu HGarcia-Lacarte MLeal Lasalle HJorquera GMartinez-Naves ECubero FJNevzorova YALamas-Paz AfalseFecal microbiota transplantation from female donors restores gut permeability and reduces liver injury and inflammation in middle-aged male mice exposed to alcohol.<h4>Background</h4>Alcohol misuse, binge drinking pattern, and gender-specific effects in the middle-aged population has been clearly underestimated. In the present study, we focused on understanding gender-specific effects of alcohol exposure on the gut-liver axis and the role of gut microbiota in modulating gender-specific responses to alcohol consumption.<h4>Methods</h4>Fifty-two-week-old female and male C57BL/6 mice were fasted for 12 h, and then administered a single oral dose of ethanol (EtOH) (6 g/kg). Controls were given a single dose of PBS. Animals were sacrificed 8 h later. Alternatively, fecal microbiota transplantation (FMT) was performed in 52-week-old male mice from female donors of the same age. Permeability of the large intestine (colon), gut microbiota, liver injury, and 2024-01-01T00:00:00Z20242026-05-26T20:06:16.116Z2026-05-26T03:07:07.403ZS-EPMC110632543869954510.3389/fnut.2024.1393014