{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Berndt A"],"funding":["NIBIB NIH HHS","NIDA NIH HHS","NIA NIH HHS","NIMH NIH HHS","NINDS NIH HHS","NIAMS NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["H<sub>2</sub>O<sub>2</sub> is a key oxidant in mammalian biology and a pleiotropic signaling molecule at the physiological level, and its excessive accumulation in conjunction with decreased cellular reduction capacity is often found to be a common pathological marker. Here, we present a red fluorescent Genetically Encoded H<sub>2</sub>O<sub>2</sub> Indicator (GEHI) allowing versatile optogenetic dissection of redox biology. Our new GEHI, oROS-HT, is a chemigenetic sensor utilizing a HaloTag and Janelia Fluor (JF) rhodamine dye as fluorescent reporters. We developed oROS-HT through a structure-guided approach aided by classic protein structures and recent protein structure prediction tools. Optimized with JF<sub>635</sub>, oROS-HT is a sensor with 635 nm excitation and 650 nm emission peak"],"journal":["Research square"],"pagination":["rs.3.rs-3974015"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11065073"],"repository":["biostudies-literature"],"pubmed_title":["Far-red and sensitive sensor for monitoring real time H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; dynamics with subcellular resolution and in multi-parametric imaging applications."],"pmcid":["PMC11065073"],"funding_grant_id":["RM1 GM131981","R01 GM139850","RF1 MH130391","U01 NS128537","T32 AG066574","R21 DA051193","F31 DA056121","T32 EB032787","P30 DA048736","P30 AR074990"],"pubmed_authors":["Moghadasi A","Smith A","Lee J","Mack D","Nance E","Bremner S","Zuniga S","Williams A","Jin Z","Gibbs C","Evitts K","Chavan V","Wait S","Boyle P","Moussavi-Harami F","Berndt A","Asencio A","Regnier M","Nguyen A","Young J"],"additional_accession":[]},"is_claimable":false,"name":"Far-red and sensitive sensor for monitoring real time H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; dynamics with subcellular resolution and in multi-parametric imaging applications.","description":"H<sub>2</sub>O<sub>2</sub> is a key oxidant in mammalian biology and a pleiotropic signaling molecule at the physiological level, and its excessive accumulation in conjunction with decreased cellular reduction capacity is often found to be a common pathological marker. Here, we present a red fluorescent Genetically Encoded H<sub>2</sub>O<sub>2</sub> Indicator (GEHI) allowing versatile optogenetic dissection of redox biology. Our new GEHI, oROS-HT, is a chemigenetic sensor utilizing a HaloTag and Janelia Fluor (JF) rhodamine dye as fluorescent reporters. We developed oROS-HT through a structure-guided approach aided by classic protein structures and recent protein structure prediction tools. Optimized with JF<sub>635</sub>, oROS-HT is a sensor with 635 nm excitation and 650 nm emission peak","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-04-13T03:18:45.013Z","creation":"2026-04-13T03:12:20.676Z"},"accession":"S-EPMC11065073","cross_references":{"pubmed":["38699332"],"doi":["10.21203/rs.3.rs-3974015/v1"]}}