biostudies-literatureXu SNational Key Research and Development Program of China2200067https://www.ebi.ac.uk/biostudies/studies/S-EPMC11065329biostudies-literatureUnknown24(5)The high salt content of food waste (FW) severely limits microbial physiological activity and reduces its biodegradability. In this study, a salt-tolerant thermophilic bacterial agent that consists of four different substrate degradation functional strains was evaluated for efficient high salt and oily FW in solid-state aerobic biodegradation disposers. The phy-chemical properties, enzyme activities, microbial community structure, and function during the biodegradation process were evaluated under high salt (5%) stress. The results showed that the agent promoted the degradation rate, increased the matrix temperature, decreased the moisture content (MC), and enhanced enzyme activities without putrid smell. High-throughput sequencing indicated community structure succession between differentEngineering in life sciencesRapid in-situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome.PMC110653292018YFA0901200Tao LHuang ZZhang XXu SWang JfalseRapid in-situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome.The high salt content of food waste (FW) severely limits microbial physiological activity and reduces its biodegradability. In this study, a salt-tolerant thermophilic bacterial agent that consists of four different substrate degradation functional strains was evaluated for efficient high salt and oily FW in solid-state aerobic biodegradation disposers. The phy-chemical properties, enzyme activities, microbial community structure, and function during the biodegradation process were evaluated under high salt (5%) stress. The results showed that the agent promoted the degradation rate, increased the matrix temperature, decreased the moisture content (MC), and enhanced enzyme activities without putrid smell. High-throughput sequencing indicated community structure succession between different2024-01-01T00:00:00Z2024 May2026-06-01T23:45:00.85Z2026-05-23T03:08:17.95ZS-EPMC110653293870841210.1002/elsc.202200067