{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hou X"],"funding":["National Natural Science Foundation of China","Natural Science Basic Research Program of Shaanxi Province"],"pagination":["103741"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11066554"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["103(6)"],"pubmed_abstract":["Fowl adenovirus serotype 4 (FAdV-4) infections result in substantial economic losses in the poultry industry. Recent findings have revealed that FAdV-4 significantly suppresses the host immune response upon infection; however, the specific viral and host factors contributing to this immunomodulatory activity remain poorly characterized. Moreover, diverse cell types exhibit differential immune responses to FAdV-4 infection. To elucidate cell-specific host responses, we performed transcriptomic analysis of FAdV-4 infected leghorn male hepatocellular (LMH) and chicken embryo fibroblast (CEF) cells. Although FAdV-4 replicated more efficiently in LMH cells, it provoked limited interferon-stimulated gene induction. In contrast, FAdV-4 infection triggered robust antiviral responses in CEF cells, including upregulation of cytosolic DNA sensing and interferon-stimulated genes. Knockdown of key cytosolic DNA sensing molecules enhanced FAdV-4 replication in LMH cells while reducing interferon-stimulated gene expression. Our findings reveal cell-specific virus-host interactions that provide insight into FAdV-4 pathogenesis while identifying factors that mediate antiviral immunity against FAdV-4."],"journal":["Poultry science"],"pubmed_title":["Differential innate immune responses to fowl adenovirus serotype 4 infection in Leghorn male hepatocellular and chicken embryo fibroblast cells."],"pmcid":["PMC11066554"],"funding_grant_id":["2023JC-QN-0190","3217284","32172843"],"pubmed_authors":["Fang J","Qi X","Wang T","Wen B","Han J","Hou X","Han S","Zhang R","Wang J","Wang L","Liu G","Chang W"],"additional_accession":[]},"is_claimable":false,"name":"Differential innate immune responses to fowl adenovirus serotype 4 infection in Leghorn male hepatocellular and chicken embryo fibroblast cells.","description":"Fowl adenovirus serotype 4 (FAdV-4) infections result in substantial economic losses in the poultry industry. Recent findings have revealed that FAdV-4 significantly suppresses the host immune response upon infection; however, the specific viral and host factors contributing to this immunomodulatory activity remain poorly characterized. Moreover, diverse cell types exhibit differential immune responses to FAdV-4 infection. To elucidate cell-specific host responses, we performed transcriptomic analysis of FAdV-4 infected leghorn male hepatocellular (LMH) and chicken embryo fibroblast (CEF) cells. Although FAdV-4 replicated more efficiently in LMH cells, it provoked limited interferon-stimulated gene induction. In contrast, FAdV-4 infection triggered robust antiviral responses in CEF cells, including upregulation of cytosolic DNA sensing and interferon-stimulated genes. Knockdown of key cytosolic DNA sensing molecules enhanced FAdV-4 replication in LMH cells while reducing interferon-stimulated gene expression. Our findings reveal cell-specific virus-host interactions that provide insight into FAdV-4 pathogenesis while identifying factors that mediate antiviral immunity against FAdV-4.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-26T20:01:26.951Z","creation":"2026-05-26T03:07:02.282Z"},"accession":"S-EPMC11066554","cross_references":{"pubmed":["38670055"],"doi":["10.1016/j.psj.2024.103741"]}}