{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["He Q"],"funding":["HHS | NIH | National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e2319727121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11067034"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(18)"],"pubmed_abstract":["The DNA sliding clamp PCNA is a multipurpose platform for DNA polymerases and many other proteins involved in DNA metabolism. The topologically closed PCNA ring needs to be cracked open and loaded onto DNA by a clamp loader, e.g., the well-studied pentameric ATPase complex RFC (RFC1-5). The CTF18-RFC complex is an alternative clamp loader found recently to bind the leading strand DNA polymerase ε and load PCNA onto leading strand DNA, but its structure and the loading mechanism have been unknown. By cryo-EM analysis of in vitro assembled human CTF18-RFC-DNA-PCNA complex, we have captured seven loading intermediates, revealing a detailed PCNA loading mechanism onto a 3'-ss/dsDNA junction by CTF18-RFC. Interestingly, the alternative loader has evolved a highly mobile CTF18 AAA+ module likely"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Cryo-EM reveals a nearly complete PCNA loading process and unique features of the human alternative clamp loader CTF18-RFC."],"pmcid":["PMC11067034"],"funding_grant_id":["GM148159","R35 GM148159","R35 GM131754","GM131754"],"pubmed_authors":["Li H","O'Donnell ME","Wang F","He Q"],"additional_accession":[]},"is_claimable":false,"name":"Cryo-EM reveals a nearly complete PCNA loading process and unique features of the human alternative clamp loader CTF18-RFC.","description":"The DNA sliding clamp PCNA is a multipurpose platform for DNA polymerases and many other proteins involved in DNA metabolism. The topologically closed PCNA ring needs to be cracked open and loaded onto DNA by a clamp loader, e.g., the well-studied pentameric ATPase complex RFC (RFC1-5). The CTF18-RFC complex is an alternative clamp loader found recently to bind the leading strand DNA polymerase ε and load PCNA onto leading strand DNA, but its structure and the loading mechanism have been unknown. By cryo-EM analysis of in vitro assembled human CTF18-RFC-DNA-PCNA complex, we have captured seven loading intermediates, revealing a detailed PCNA loading mechanism onto a 3'-ss/dsDNA junction by CTF18-RFC. Interestingly, the alternative loader has evolved a highly mobile CTF18 AAA+ module likely","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-01T05:37:45.611Z","creation":"2026-04-08T09:41:18.994Z"},"accession":"S-EPMC11067034","cross_references":{"pubmed":["38669181"],"doi":["10.1073/pnas.2319727121"]}}