biostudies-literatureUnknown27(5)Brinkkemper MBill & Melinda Gates FoundationZoonotic events by sarbecoviruses have sparked an epidemic (severe acute respiratory syndrome coronavirus [SARS-CoV]) and a pandemic (SARS-CoV-2) in the past two decades. The continued risk of spillovers from animals to humans is an ongoing threat to global health and a pan-sarbecovirus vaccine would be an important contribution to pandemic preparedness. Here, we describe multivalent virosome-based vaccines that present stabilized spike proteins from four sarbecovirus strains, one from each clade. A cocktail of four monovalent virosomes or a mosaic virosome preparation induced broad sarbecovirus binding and neutralizing antibody responses in mice. Pre-existing immunity against SARS-CoV-2 and extending the intervals between immunizations enhanced antibody responses. These results should infiScience109719https://www.ebi.ac.uk/biostudies/studies/S-EPMC11068555biostudies-literatureA spike virosome vaccine induces pan-sarbecovirus antibody responses in mice.PMC11068555Poniman MSanders RWBijl TPLBhoelan FIddouch WABrinkkemper Mvan Gils MJGuerra DSiteur-van Rijnstra EBemelman DKempers Rvan der Velden YUGrobben MTejjani KStegmann TSliepen KfalseA spike virosome vaccine induces pan-sarbecovirus antibody responses in mice.Zoonotic events by sarbecoviruses have sparked an epidemic (severe acute respiratory syndrome coronavirus [SARS-CoV]) and a pandemic (SARS-CoV-2) in the past two decades. The continued risk of spillovers from animals to humans is an ongoing threat to global health and a pan-sarbecovirus vaccine would be an important contribution to pandemic preparedness. Here, we describe multivalent virosome-based vaccines that present stabilized spike proteins from four sarbecovirus strains, one from each clade. A cocktail of four monovalent virosomes or a mosaic virosome preparation induced broad sarbecovirus binding and neutralizing antibody responses in mice. Pre-existing immunity against SARS-CoV-2 and extending the intervals between immunizations enhanced antibody responses. These results should inf2024-01-01T00:00:00Z2024 May2026-06-01T21:21:01.823Z2026-05-21T03:08:21.383ZS-EPMC110685553870684810.1016/j.isci.2024.109719