biostudies-literatureWang ZNational Key Research and Development Program of China41https://www.ebi.ac.uk/biostudies/studies/S-EPMC11070108biostudies-literatureUnknown23(1)<h4>Background</h4>Infections caused by linezolid-resistant enterococci (LRE) are clinically difficult to treat and threaten patient health. However, there is a lack of studies on long time-span LRE strains in China. For this reason, our study comprehensively revealed the resistance mechanisms of LRE strains collected in a Chinese tertiary care hospital from 2011 to 2022.<h4>Methods</h4>Enterococcal strains were screened and verified after retrospective analysis of microbial data. Subsequently, 65 LRE strains (61 Enterococcus faecalis and 4 Enterococcus faecium, MIC ≥ 8 µg/ml), 1 linezolid-intermediate Enterococcus faecium (MIC = 4 µg/ml) and 1 linezolid-susceptible Enterococcus faecium (MIC = 1.5 µg/ml) were submitted for whole-genome sequencing (WGS) analysis and bioinformatics analysis.Annals of clinical microbiology and antimicrobialsGenomic epidemiology reveals multiple mechanisms of linezolid resistance in clinical enterococci in China.PMC110701082021YFF0703805Hu SWu LLiu LZhang JZhang ZHe ZLiu CLiu DSun HWang ZfalseGenomic epidemiology reveals multiple mechanisms of linezolid resistance in clinical enterococci in China.<h4>Background</h4>Infections caused by linezolid-resistant enterococci (LRE) are clinically difficult to treat and threaten patient health. However, there is a lack of studies on long time-span LRE strains in China. For this reason, our study comprehensively revealed the resistance mechanisms of LRE strains collected in a Chinese tertiary care hospital from 2011 to 2022.<h4>Methods</h4>Enterococcal strains were screened and verified after retrospective analysis of microbial data. Subsequently, 65 LRE strains (61 Enterococcus faecalis and 4 Enterococcus faecium, MIC ≥ 8 µg/ml), 1 linezolid-intermediate Enterococcus faecium (MIC = 4 µg/ml) and 1 linezolid-susceptible Enterococcus faecium (MIC = 1.5 µg/ml) were submitted for whole-genome sequencing (WGS) analysis and bioinformatics analysis.2024-01-01T00:00:00Z2024 May2026-04-08T15:57:06.88Z2026-04-08T05:45:06.248ZS-EPMC110701083870457710.1186/s12941-024-00689-0