biostudies-literatureEl-Meihy RMDeanship of Scientific Research, King Khalid University104002https://www.ebi.ac.uk/biostudies/studies/S-EPMC11070271biostudies-literatureUnknown31(6)American foulbrood (AFB) is a harmful honeybee disease primarily caused by <i>Paenibacillus larvae</i>. The study aims to isolate and identify the AFB causative agent <i>P. larvae</i> and their specific phages to use as a new biological method for AFB disease control. Eight apiaries were inspected for AFB infections. Symptoms of diseased brood comb, were odd brood cells with soft brown decayed brood amongst healthy brood, were identified in the field and demonstrated the prevalence of AFB in every apiary. Three <i>P. larvae</i> isolates were identified using traditional techniques using a 452-bp PCR amplicon specific to the bacterial 16SrRNA gene and was compared between <i>Paenibacillus</i> isolates. Additionally, specific phages of <i>P. larvae</i> strains were applied to examine their eSaudi journal of biological sciencesProbing the interaction of <i>Paenibacillus larvae</i> bacteriophage as a biological agent to control the american foulbrood disease in honeybee.PMC11070271RGP2/ 227/44Hassan EOAl-Ghamdi MSNowar EENegm SEl-Meihy RMAlamoudi SAAl-Hoshani NfalseProbing the interaction of <i>Paenibacillus larvae</i> bacteriophage as a biological agent to control the american foulbrood disease in honeybee.American foulbrood (AFB) is a harmful honeybee disease primarily caused by <i>Paenibacillus larvae</i>. The study aims to isolate and identify the AFB causative agent <i>P. larvae</i> and their specific phages to use as a new biological method for AFB disease control. Eight apiaries were inspected for AFB infections. Symptoms of diseased brood comb, were odd brood cells with soft brown decayed brood amongst healthy brood, were identified in the field and demonstrated the prevalence of AFB in every apiary. Three <i>P. larvae</i> isolates were identified using traditional techniques using a 452-bp PCR amplicon specific to the bacterial 16SrRNA gene and was compared between <i>Paenibacillus</i> isolates. Additionally, specific phages of <i>P. larvae</i> strains were applied to examine their e2024-01-01T00:00:00Z2024 Jun2026-05-29T17:47:18.215Z2025-08-17T03:05:45.408ZS-EPMC110702713870671910.1016/j.sjbs.2024.104002